Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16078
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dc.contributor.authorMartens, Thomas F.-
dc.contributor.authorVercauteren, Dries-
dc.contributor.authorForier, Katrien-
dc.contributor.authorDeschout, Hendrik-
dc.contributor.authorRemaut, Katrien-
dc.contributor.authorPAESEN, Rik-
dc.contributor.authorAMELOOT, Marcel-
dc.contributor.authorEngbersen, Johan F. J.-
dc.contributor.authorDemeester, Jo-
dc.contributor.authorDe Smedt, Stefaan C.-
dc.contributor.authorBraeckmans, Kevin-
dc.date.accessioned2013-12-20T10:13:30Z-
dc.date.available2013-12-20T10:13:30Z-
dc.date.issued2013-
dc.identifier.citationNANOMEDICINE, 8 (12), p. 1955-1968-
dc.identifier.issn1743-5889-
dc.identifier.urihttp://hdl.handle.net/1942/16078-
dc.description.abstractAim: To develop a robust assay to evaluate and compare the intravitreal mobility of nanoparticles in the intact vitreous body. Materials & methods: Excised bovine eyes were prepared to preserve the fragile structure of the vitreous humor, while permitting high-resolution fluorescence microscopy and single-particle tracking analysis of intravitreally injected nanoparticles. This assay was validated by analyzing polystyrene beads and further employed to evaluate gene nanomedicines composed of poly(amido amine)s and plasmid DNA. Results: The assay was able to distinguish immobilized cationic nanoparticles from mobile PEGylated nanoparticles. PEGylation of the polyplexes resulted in a drastic improvement of their mobility. Conclusion: An ex vivo eye model is presented for studying nanoparticle mobility in intact vitreous humor by single-particle tracking microscopy. These results give important guidelines for developing gene- and drug-delivery nanomedicines that are compatible with intravitreal administration. Original submitted 20 April 2012; Revised submitted 22 November 2012-
dc.description.sponsorshipFinancial support by the Ghent University Special Research Fund, the Fund for Scientific Research Flanders (FWO, Belgium), Hasselt University and the Interreg program is acknowledged with gratitude. D Vercauteren is a Doctoral Fellow of the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT), Belgium. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.-
dc.language.isoen-
dc.subject.otherdiffusion; intravitreal injection; nanomedicine; nanoparticle; ocular gene therapy; polyplex; single-particle tracking-
dc.titleMeasuring the intravitreal mobility of nanomedicines with single-particle tracking microscopy-
dc.typeJournal Contribution-
dc.identifier.epage1968-
dc.identifier.issue12-
dc.identifier.spage1955-
dc.identifier.volume8-
local.bibliographicCitation.jcatA1-
dc.description.notesDe Smedt, SC (reprint author), De Smedt, SC (reprint author) De Smedt, SC (reprint author)-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.identifier.doi10.2217/nnm.12.202-
dc.identifier.isi000327379600009-
item.fulltextNo Fulltext-
item.fullcitationMartens, Thomas F.; Vercauteren, Dries; Forier, Katrien; Deschout, Hendrik; Remaut, Katrien; PAESEN, Rik; AMELOOT, Marcel; Engbersen, Johan F. J.; Demeester, Jo; De Smedt, Stefaan C. & Braeckmans, Kevin (2013) Measuring the intravitreal mobility of nanomedicines with single-particle tracking microscopy. In: NANOMEDICINE, 8 (12), p. 1955-1968.-
item.contributorMartens, Thomas F.-
item.contributorVercauteren, Dries-
item.contributorForier, Katrien-
item.contributorDeschout, Hendrik-
item.contributorRemaut, Katrien-
item.contributorPAESEN, Rik-
item.contributorAMELOOT, Marcel-
item.contributorEngbersen, Johan F. J.-
item.contributorDemeester, Jo-
item.contributorDe Smedt, Stefaan C.-
item.contributorBraeckmans, Kevin-
item.validationecoom 2014-
item.accessRightsClosed Access-
crisitem.journal.issn1743-5889-
crisitem.journal.eissn1748-6963-
Appears in Collections:Research publications
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