Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16095
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dc.contributor.authorVANBRABANT, Jeroen-
dc.contributor.authorLEIRS, Karen-
dc.contributor.authorVANSCHOENBEEK, Katrijn-
dc.contributor.authorLAMMERTYN, Jeroen-
dc.contributor.authorMICHIELS, Luc-
dc.date.accessioned2013-12-24T11:19:09Z-
dc.date.available2013-12-24T11:19:09Z-
dc.date.issued2014-
dc.identifier.citationANALYST, 139 (3), p. 589-595-
dc.identifier.issn0003-2654-
dc.identifier.urihttp://hdl.handle.net/1942/16095-
dc.description.abstractCurrent aptamer selection procedures enable limited control and transparency on how the DNA selection pool is evolving. Affinity tests and binding analysis are not always as informative. Here we show that real-time PCR provides a valuable tool for the follow-up of aptamer selection. Limited time, work and amount of amplified ssDNA make this an interesting instrument to set-up a SELEX design and monitor the enrichment of oligonucleotides. reMelting Curve Analysis (rMCA) after reannealing at stringent conditions provides information about enrichment, compared to a random library. Monitoring the SELEX process and optimizing conditions by means of the proposed methods can increase the selection efficiency in a controlled way. rMCA is applied in enrichment simulations and three different selection procedures. Our results imply that rMCA can be used for different SELEX designs and different targets. SELEX pool diversity analysis by rMCA is proven to be a useful, reproducible tool to detect and evaluate enrichment of specific binding aptamers while the selection procedure is performed.-
dc.description.sponsorshipThis work was supported by the Limburg Sterk Merk [tUL impulsfinanciering phase II to V.J]; the European union through EFRO, Flemish government and Province of Limburg [NanoSensEu grant number IVA-VLANED 1.44]; and the KU Leuven (OT/13/058).-
dc.language.isoen-
dc.rights© Royal Society of Chemistry 2014-
dc.subject.otherAptamers; SELEX; enrichment monitoring; real-time PCR-
dc.titlereMelting Curve Analysis as a Tool for Enrichment Monitoring in the SELEX Process.-
dc.typeJournal Contribution-
dc.identifier.epage595-
dc.identifier.issue3-
dc.identifier.spage589-
dc.identifier.volume139-
local.bibliographicCitation.jcatA1-
dc.description.notesVanbrabant, J (reprint author),Univ Hasselt, BIOMED, B-3590 Diepenbeek, Belgium, 1jeroenvanbrabant@gmail.com; luc.michiels@uhasselt.be-
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local.type.refereedRefereed-
local.type.specifiedArticle-
dc.identifier.doi10.1039/C3AN01884A-
dc.identifier.isi000328912000009-
dc.identifier.urlhttp://pubs.rsc.org/en/content/articlelanding/2013/an/c3an01884a#!divAbstract-
item.contributorVANBRABANT, Jeroen-
item.contributorLEIRS, Karen-
item.contributorVANSCHOENBEEK, Katrijn-
item.contributorLAMMERTYN, Jeroen-
item.contributorMICHIELS, Luc-
item.validationecoom 2015-
item.fullcitationVANBRABANT, Jeroen; LEIRS, Karen; VANSCHOENBEEK, Katrijn; LAMMERTYN, Jeroen & MICHIELS, Luc (2014) reMelting Curve Analysis as a Tool for Enrichment Monitoring in the SELEX Process.. In: ANALYST, 139 (3), p. 589-595.-
item.accessRightsOpen Access-
item.fulltextWith Fulltext-
crisitem.journal.issn0003-2654-
crisitem.journal.eissn1364-5528-
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