Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/16095
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | VANBRABANT, Jeroen | - |
dc.contributor.author | LEIRS, Karen | - |
dc.contributor.author | VANSCHOENBEEK, Katrijn | - |
dc.contributor.author | LAMMERTYN, Jeroen | - |
dc.contributor.author | MICHIELS, Luc | - |
dc.date.accessioned | 2013-12-24T11:19:09Z | - |
dc.date.available | 2013-12-24T11:19:09Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | ANALYST, 139 (3), p. 589-595 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.uri | http://hdl.handle.net/1942/16095 | - |
dc.description.abstract | Current aptamer selection procedures enable limited control and transparency on how the DNA selection pool is evolving. Affinity tests and binding analysis are not always as informative. Here we show that real-time PCR provides a valuable tool for the follow-up of aptamer selection. Limited time, work and amount of amplified ssDNA make this an interesting instrument to set-up a SELEX design and monitor the enrichment of oligonucleotides. reMelting Curve Analysis (rMCA) after reannealing at stringent conditions provides information about enrichment, compared to a random library. Monitoring the SELEX process and optimizing conditions by means of the proposed methods can increase the selection efficiency in a controlled way. rMCA is applied in enrichment simulations and three different selection procedures. Our results imply that rMCA can be used for different SELEX designs and different targets. SELEX pool diversity analysis by rMCA is proven to be a useful, reproducible tool to detect and evaluate enrichment of specific binding aptamers while the selection procedure is performed. | - |
dc.description.sponsorship | This work was supported by the Limburg Sterk Merk [tUL impulsfinanciering phase II to V.J]; the European union through EFRO, Flemish government and Province of Limburg [NanoSensEu grant number IVA-VLANED 1.44]; and the KU Leuven (OT/13/058). | - |
dc.language.iso | en | - |
dc.rights | © Royal Society of Chemistry 2014 | - |
dc.subject.other | Aptamers; SELEX; enrichment monitoring; real-time PCR | - |
dc.title | reMelting Curve Analysis as a Tool for Enrichment Monitoring in the SELEX Process. | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 595 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 589 | - |
dc.identifier.volume | 139 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Vanbrabant, J (reprint author),Univ Hasselt, BIOMED, B-3590 Diepenbeek, Belgium, 1jeroenvanbrabant@gmail.com; luc.michiels@uhasselt.be | - |
dc.relation.references | 1 R. Stoltenburg, C. Reinemann and B. Strehlitz, Biomol. Eng., 2008, 4, 381-403. 2 J. Müller, O. El-Maarri, J. Oldeburg, B. Pötzsch and G. Mayer, Anal. Bioanal. Chem., 2008, 390, 1033–1037. 3 W. Yoshida, E. Mochizuki, M. Takase, H. Hasegawa, Y. Moritaa, H. Yamazakib, K. Sodea and K. Ikebukuroa, Biosens. Bioelectron., 2009, 24 (5), 1116-1120. 4 C. Wang, G. Yang, Z. Luo and H. Ding, Acta. Biochim. Biophys. Sin., 2009, 41 (4), 335-340. 5 X. Cao, S. Li, L. Chen, H. Ding, H. Xu, Y. Huang, J. Li, N. Liu, W. Cao, Y. Zhu, B. Shen and N. Shao, Nucleic Acids Res., 2009, 37, 4621–4628. 6 B. Hwang and S.I. Lee, Biochem. Biophys. Res. Commun., 2002, 290, 656-662. 7 M. Berezovski, A. Drabovich, S.M. Krylova, M. Musheev, V. Okhonin, A. Petrov and S.N. Krylov, J. Am. Chem. Soc., 2005, 127, 3165-3171. 8 E. Dausse, S. Taouji, L. Evadé, C. Di Primo, E. Chevet and J. Toulmé, J. Nanobiotechnology, 2011, 9 (25), DOI: 10.1186/1477-3155-9-25 9 T. Tran, K. Knez, K. Janssen, J. Pollet, D. Spasic and J. Lammertyn, Biosens. Bioelectron., 2013, 43, 245-251. 10 T. Tran, K. Janssen, J. Pollet, E. Lammertyn, J. Anné, A. Van Schepdael and J. Lammertyn, Molecules, 2010, 15, 1127-1140. 11 W. Rowe, M. Platt and P.J.R. Day, Integr. Biol., 2009, 1, 53-58. 12 J.H. Niazi, S.J. Lee, Y.S. Kim and M.B. Gu, Bioorg. Med. Chem., 2008, 1, 1254-1261. 13 J. Charlton and D. Smith, RNA, 1999, 5, 1326-1332. 14 R.J. Britten and D.E. Kohne, Science, 1968, 161, 529–540. 15 P.D. Baum and J.M. McCune, Nat. Methods, 2006, 3, 895-901. 16 T. Schütze, P.F. Arndt, M. Menger, A. Wochner, M. Vingron, A.E. Volker, H. Lehrach, C. Kaps and J. Glökler, Nucleic Acids Res., 2010, 38 (4), e2. DOI: 10.1093/nar/gkp1108 17 T.W. Wiegand, P.B. Williams, S.C. Dreskin, M.H. Jouvin, J.P. Kinet and D. Tasset, J. Immunol., 1996, 157, 221–230. 18 R.J. Britten, D.E. Graham and B.R. Neufeld, Methods Enzymol., 1974, 29, 363–418. 19 D. Lamoureux, D.G. Peterson, W. Li, J.P. Fellers and B.S. Gill, Genome, 2005, 48 (6), 1120–1126. 20 M. Tsujimoto, K. Inoue and S. Nojima, J. Biochem, 1983, 5, 1367-1373. 21 M.J. O'Neil, (ed.) The Merck Index - An Encyclopedia of Chemicals, Drugs, and Biologicals. Merck & Co. Inc., Whitehouse Station, N.J., USA, 14nd edn., 2006. | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1039/C3AN01884A | - |
dc.identifier.isi | 000328912000009 | - |
dc.identifier.url | http://pubs.rsc.org/en/content/articlelanding/2013/an/c3an01884a#!divAbstract | - |
item.contributor | VANBRABANT, Jeroen | - |
item.contributor | LEIRS, Karen | - |
item.contributor | VANSCHOENBEEK, Katrijn | - |
item.contributor | LAMMERTYN, Jeroen | - |
item.contributor | MICHIELS, Luc | - |
item.validation | ecoom 2015 | - |
item.fullcitation | VANBRABANT, Jeroen; LEIRS, Karen; VANSCHOENBEEK, Katrijn; LAMMERTYN, Jeroen & MICHIELS, Luc (2014) reMelting Curve Analysis as a Tool for Enrichment Monitoring in the SELEX Process.. In: ANALYST, 139 (3), p. 589-595. | - |
item.accessRights | Open Access | - |
item.fulltext | With Fulltext | - |
crisitem.journal.issn | 0003-2654 | - |
crisitem.journal.eissn | 1364-5528 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
J. Vanbrabant et al - 2014 - rMCA as a tool for enrichment monitoring in the SELEX process.pdf | Published version | 702.19 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
20
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
26
checked on Apr 22, 2024
Page view(s)
70
checked on Sep 7, 2022
Download(s)
254
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.