Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16120
Title: Variability in contrast agent uptake by different but similar stem cell types
Authors: Ketkar-Atre, Ashwini
STRUYS, Tom 
Soenen, Stefaan J.
LAMBRICHTS, Ivo 
Verfaillie, Catherine M.
De Cuyper, Marcel
Himmelreich, Uwe
Issue Date: 2013
Source: INTERNATIONAL JOURNAL OF NANOMEDICINE, 8, p. 4577-4591
Abstract: The need to track and evaluate the fate of transplanted cells is an important issue in regenerative medicine. In order to accomplish this, pre-labelling cells with magnetic resonance imaging (MRI) contrast agents is a well-established method. Uptake of MRI contrast agents by non-phagocytic stem cells, and factors such as cell homeostasis or the adverse effects of contrast agents on cell biology have been extensively studied, but in the context of nanoparticle (NP)-specific parameters. Here, we have studied three different types of NPs (Endorem (R), magnetoliposomes [MLs], and citrate coated C-200) to label relatively larger, mesenchymal stem cells (MSCs) and, much smaller yet faster proliferating, multipotent adult progenitor cells (MAPCs). Both cell types are similar, as they are isolated from bone marrow and have substantial regenerative potential, which make them interesting candidates for comparative experiments. Using NPs with different surface coatings and sizes, we found that differences in the proliferative and morphological characteristics of the cells used in the study are mainly responsible for the fate of endocytosed iron, intracellular iron concentration, and cytotoxic responses. The quantitative analysis, using high-resolution electron microscopy images, demonstrated a strong relationship between cell volume/surface, uptake, and cytotoxicity. Interestingly, uptake and toxicity trends are reversed if intracellular concentrations, and not amounts, are considered. This indicates that more attention should be paid to cellular parameters such as cell size and proliferation rate in comparative cell-labeling studies.
Notes: Himmelreich, U (reprint author), Katholieke Univ Leuven, Biomed MRI Unit MoSAIC, O&N1,BUS 505,Herestr 49, B-3000 Louvain, Belgium, uwe.himmelreich@med.kuleuven.be
Keywords: cell labeling; MR contrast agents; transmission electron microscopy; mesenchymal stem cells; multipotent adult progenitor cells; magnetic resonance imaging; nanoparticles; iron oxide
Document URI: http://hdl.handle.net/1942/16120
ISSN: 1178-2013
e-ISSN: 1178-2013
DOI: 10.2147/IJN.S51588
ISI #: 000327709200001
Category: A1
Type: Journal Contribution
Validations: ecoom 2014
Appears in Collections:Research publications

Files in This Item:
File Description SizeFormat 
ketkar-atre 1.pdfpublished version5 MBAdobe PDFView/Open
Show full item record

SCOPUSTM   
Citations

16
checked on Sep 7, 2020

WEB OF SCIENCETM
Citations

17
checked on May 20, 2022

Page view(s)

82
checked on May 23, 2022

Download(s)

118
checked on May 23, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.