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http://hdl.handle.net/1942/16135
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DC Field | Value | Language |
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dc.contributor.author | TA, Duy Tien | - |
dc.contributor.author | STEEN REDEKER, Erik | - |
dc.contributor.author | GUEDENS, Wanda | - |
dc.contributor.author | ADRIAENSENS, Peter | - |
dc.date.accessioned | 2014-01-14T14:06:44Z | - |
dc.date.available | 2014-01-14T14:06:44Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Engineering of Functional Interfaces 2013, Hasselt, 8-9/7/2013 | - |
dc.identifier.uri | http://hdl.handle.net/1942/16135 | - |
dc.description.abstract | The conserved LPETG motif, at which sortase A-catalyzed transpeptidation occurs, is engineered at the C-terminal region of the variable domain of single-domain heavy chain antibody (or nanobody) against Vascular Cell Adhesion Molecule 1 (VCAM1). The recombinant nanobody can then subsequently be ligated, under sortase A catalysis, to a variety of oligoglycine containing targets, including material surfaces, contrast labeling molecules and molecules functionalized for specific chemical coupling reactions. | - |
dc.description.sponsorship | This work is supported by the FWO project G.0581.12N. We thank Prof. Dr. Muyldermans (VUB/VIB) for kindly supplying the vector pHEN6(c)-VCAM1 encoding the nanobody. | - |
dc.language.iso | en | - |
dc.subject.other | nanobody, sortase A, surface coupling, VCAM1 | - |
dc.title | Sortase A-mediated functionalization of nanobodies toward surface coupling | - |
dc.type | Conference Material | - |
local.bibliographicCitation.conferencedate | 8-9/7/2013 | - |
local.bibliographicCitation.conferencename | Engineering of Functional Interfaces 2013 | - |
local.bibliographicCitation.conferenceplace | Hasselt | - |
local.bibliographicCitation.jcat | C2 | - |
dc.relation.references | [1] R. G. Kruger et al., Biochemistry 43, 1541 – 1551 (2004). [2] M. Dumoulin et al., Protein Sci 11 (3), 500 – 515 (2002). [3] T. Proft, Biotechnol Lett 32, 1 – 10 (2010). | - |
local.type.refereed | Refereed | - |
local.type.specified | Poster | - |
item.accessRights | Open Access | - |
item.fullcitation | TA, Duy Tien; STEEN REDEKER, Erik; GUEDENS, Wanda & ADRIAENSENS, Peter (2013) Sortase A-mediated functionalization of nanobodies toward surface coupling. In: Engineering of Functional Interfaces 2013, Hasselt, 8-9/7/2013. | - |
item.fulltext | With Fulltext | - |
item.contributor | TA, Duy Tien | - |
item.contributor | STEEN REDEKER, Erik | - |
item.contributor | GUEDENS, Wanda | - |
item.contributor | ADRIAENSENS, Peter | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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Poster_Tien_final.pdf | Conference material | 240.53 kB | Adobe PDF | View/Open |
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