Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16135
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dc.contributor.authorTA, Duy Tien-
dc.contributor.authorSTEEN REDEKER, Erik-
dc.contributor.authorGUEDENS, Wanda-
dc.contributor.authorADRIAENSENS, Peter-
dc.date.accessioned2014-01-14T14:06:44Z-
dc.date.available2014-01-14T14:06:44Z-
dc.date.issued2013-
dc.identifier.citationEngineering of Functional Interfaces 2013, Hasselt, 8-9/7/2013-
dc.identifier.urihttp://hdl.handle.net/1942/16135-
dc.description.abstractThe conserved LPETG motif, at which sortase A-catalyzed transpeptidation occurs, is engineered at the C-terminal region of the variable domain of single-domain heavy chain antibody (or nanobody) against Vascular Cell Adhesion Molecule 1 (VCAM1). The recombinant nanobody can then subsequently be ligated, under sortase A catalysis, to a variety of oligoglycine containing targets, including material surfaces, contrast labeling molecules and molecules functionalized for specific chemical coupling reactions.-
dc.description.sponsorshipThis work is supported by the FWO project G.0581.12N. We thank Prof. Dr. Muyldermans (VUB/VIB) for kindly supplying the vector pHEN6(c)-VCAM1 encoding the nanobody.-
dc.language.isoen-
dc.subject.othernanobody, sortase A, surface coupling, VCAM1-
dc.titleSortase A-mediated functionalization of nanobodies toward surface coupling-
dc.typeConference Material-
local.bibliographicCitation.conferencedate8-9/7/2013-
local.bibliographicCitation.conferencenameEngineering of Functional Interfaces 2013-
local.bibliographicCitation.conferenceplaceHasselt-
local.bibliographicCitation.jcatC2-
dc.relation.references[1] R. G. Kruger et al., Biochemistry 43, 1541 – 1551 (2004). [2] M. Dumoulin et al., Protein Sci 11 (3), 500 – 515 (2002). [3] T. Proft, Biotechnol Lett 32, 1 – 10 (2010).-
local.type.refereedRefereed-
local.type.specifiedPoster-
item.accessRightsOpen Access-
item.fullcitationTA, Duy Tien; STEEN REDEKER, Erik; GUEDENS, Wanda & ADRIAENSENS, Peter (2013) Sortase A-mediated functionalization of nanobodies toward surface coupling. In: Engineering of Functional Interfaces 2013, Hasselt, 8-9/7/2013.-
item.fulltextWith Fulltext-
item.contributorTA, Duy Tien-
item.contributorSTEEN REDEKER, Erik-
item.contributorGUEDENS, Wanda-
item.contributorADRIAENSENS, Peter-
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