Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/16189
Title: | In vivo hepatocyte MR imaging using lactose functionalized magnetoliposomes | Authors: | Ketkar-Atre, Ashwini STRUYS, Tom Dresselaers, Tom Hodenius, Michael Mannaerts, Inge Ni, Yicheng LAMBRICHTS, Ivo Van Grunsven, Leo A. De Cuyper, Marcel Himmelreich, Uwe |
Issue Date: | 2014 | Source: | BIOMATERIALS, 35 (3), p. 1015-1024 | Abstract: | The aim of this study was to assess a novel lactose functionalized magnetoliposomes (MLs) as an MR contrast agent to target hepatocytes as well as to evaluate the targeting ability of MLs for in vivo applications. In the present work, 17 nm sized iron oxide cores functionalized with anionic MLs bearing lactose moieties were used for targeting the asialoglycoprotein receptor (ASGP-r), which is highly expressed in hepatocytes. Non-functionalized anionic MLs were tested as negative controls. The size distribution of lactose and anionic MLs was determined by transmission electron microscopy (TEM) and dynamic light scattering (DLS). After intravenous administration of both MLs, contrast enhancement in the liver was observed by magnetic resonance imaging (MRI). Label retention was monitored non-invasively by MRI and validated with Prussian blue staining and TEM for up to eight days post MLs administration. Although the MRI signal intensity did not show significant differences between functionalized and non-functionalized particles, iron-specific Prussian blue staining and TEM analysis confirmed the uptake of lactose MLs mainly in hepatocytes. In contrast, non-functionalized anionic MLs were mainly taken up by Kupffer and sinusoidal cells. Target specificity was further confirmed by high-resolution MR imaging of phantoms containing isolated hepatocytes, Kupffer cell (KCs) and hepatic stellate cells (HSCs) fractions. Hypointense signal was observed for hepatocytes isolated from animals which received lactose MLs but not from animals which received anionic MLs. These data demonstrate that galactose-functionalized MLs can be used as a hepatocyte targeting MR contrast agent to potentially aid in the diagnosis of hepatic diseases if the non-specific uptake by KCs is taken into account. (C) 2013 Elsevier Ltd. All rights reserved. | Keywords: | Asialoglycoprotein receptor (ASGPr-1); Hepatocyte; Liver; MRI; Lactose; Magnetoliposomes | Document URI: | http://hdl.handle.net/1942/16189 | ISSN: | 0142-9612 | e-ISSN: | 1878-5905 | DOI: | 10.1016/j.biomaterials.2013.10.029 | ISI #: | 000328806900014 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2015 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
ketkar-atre 1.pdf Restricted Access | Published version | 4.12 MB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
29
checked on Sep 5, 2020
WEB OF SCIENCETM
Citations
28
checked on Oct 14, 2024
Page view(s)
60
checked on Aug 31, 2022
Download(s)
46
checked on Aug 31, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.