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http://hdl.handle.net/1942/1626
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DC Field | Value | Language |
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dc.contributor.author | CHRISTIAENS, Pieter | - |
dc.contributor.author | VERMEEREN, Veronique | - |
dc.contributor.author | WENMACKERS, Sylvia | - |
dc.contributor.author | DAENEN, Michael | - |
dc.contributor.author | HAENEN, Ken | - |
dc.contributor.author | NESLADEK, Milos | - |
dc.contributor.author | VAN DE VEN, Martin | - |
dc.contributor.author | AMELOOT, Marcel | - |
dc.contributor.author | MICHIELS, Luc | - |
dc.contributor.author | WAGNER, Patrick | - |
dc.date.accessioned | 2007-06-15T09:10:26Z | - |
dc.date.available | 2007-06-15T09:10:26Z | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, 22(2). p. 170-177 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/1942/1626 | - |
dc.description.abstract | Chemical vapour deposited (CVD) diamond is a very promising material for biosensor fabrication owing both to its chemical inertness and the ability to make it electrical semiconducting that allows for connection with integrated circuits. For biosensor construction, a biochemical method to immobilize nucleic acids to a diamond surface has been developed. Nanocrystalline diamond is grown using microwave plasma-enhanced chemical vapour deposition (MPECVD). After hydrogenation of the surface, 10-undecenoic acid, an ω-unsaturated fatty acid, is tethered by 254 nm photochemical attachment. This is followed by 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide (EDC)-mediated attachment of amino (NH2)-modified dsDNA. The functionality of the covalently bound dsDNA molecules is confirmed by fluorescence measurements, PCR and gel electrophoresis during 35 denaturation and rehybridisation steps. The linking method after the fatty acid attachment can easily be applied to other biomolecules like antibodies and enzymes. | - |
dc.language.iso | en | - |
dc.publisher | Elsevier | - |
dc.subject.other | DNA immobilization; Rehybridisation; Affinity biosensors; Nanocrystalline diamond; Fatty acids; EDC coupling; Amide bond formation | - |
dc.title | EDC-mediated DNA attachment to nanocrystalline CVD diamond films | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 177 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 170 | - |
dc.identifier.volume | 22 | - |
local.bibliographicCitation.jcat | A1 | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1016/j.bios.2005.12.013 | - |
dc.identifier.isi | 000240906100002 | - |
item.fulltext | No Fulltext | - |
item.fullcitation | CHRISTIAENS, Pieter; VERMEEREN, Veronique; WENMACKERS, Sylvia; DAENEN, Michael; HAENEN, Ken; NESLADEK, Milos; VAN DE VEN, Martin; AMELOOT, Marcel; MICHIELS, Luc & WAGNER, Patrick (2006) EDC-mediated DNA attachment to nanocrystalline CVD diamond films. In: BIOSENSORS & BIOELECTRONICS, 22(2). p. 170-177. | - |
item.validation | ecoom 2007 | - |
item.accessRights | Closed Access | - |
item.contributor | CHRISTIAENS, Pieter | - |
item.contributor | VERMEEREN, Veronique | - |
item.contributor | WENMACKERS, Sylvia | - |
item.contributor | DAENEN, Michael | - |
item.contributor | HAENEN, Ken | - |
item.contributor | NESLADEK, Milos | - |
item.contributor | VAN DE VEN, Martin | - |
item.contributor | AMELOOT, Marcel | - |
item.contributor | MICHIELS, Luc | - |
item.contributor | WAGNER, Patrick | - |
crisitem.journal.issn | 0956-5663 | - |
crisitem.journal.eissn | 1873-4235 | - |
Appears in Collections: | Research publications |
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