Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/1628
Title: Gel-free analysis of the human brain proteome: Application of liquid chromatography and mass spectrometry on biopsy and autopsy samples
Authors: DUMONT, Debora 
NOBEN, Jean-Paul 
VERHAERT, Peter 
STINISSEN, Piet 
ROBBEN, Johan 
Issue Date: 2006
Publisher: Wiley
Source: PROTEOMICS, 60(18). p. 4967-4977
Abstract: This paper reports on the findings of the Biomedical Research Institute, as one of the participants in the pilot study of the HUPO Brain Proteome Project. A biopsy and autopsy study sample derived from human brain was distributed among the participants for proteomic analysis. In our laboratory, attention was focused on protein identification using the bottom-up shotgun approach. Protein extracts derived from both samples were trypsinized and analyzed separately by 2-D LC and MS. In a complementary approach, the tryptic digests were analyzed directly by LC-ESI-MS/MS and gas-phase fractionation in the mass spectrometer. Taken together, both proteomic approaches in combination with a stringent evaluation process, resulted in the confident identification of 209 proteins in the human brain samples under investigation.
Keywords: Gas-phase fractionation • HUPO Brain Proteome Project • Two-dimensional liquid chromatography • Validation
Document URI: http://hdl.handle.net/1942/1628
ISSN: 1615-9853
e-ISSN: 1615-9861
DOI: 10.1002/pmic.200600080
ISI #: 000240875500008
Category: A1
Type: Journal Contribution
Validations: ecoom 2007
Appears in Collections:Research publications

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