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Title: | Gel-free analysis of the human brain proteome: Application of liquid chromatography and mass spectrometry on biopsy and autopsy samples | Authors: | DUMONT, Debora NOBEN, Jean-Paul VERHAERT, Peter STINISSEN, Piet ROBBEN, Johan |
Issue Date: | 2006 | Publisher: | Wiley | Source: | PROTEOMICS, 60(18). p. 4967-4977 | Abstract: | This paper reports on the findings of the Biomedical Research Institute, as one of the participants in the pilot study of the HUPO Brain Proteome Project. A biopsy and autopsy study sample derived from human brain was distributed among the participants for proteomic analysis. In our laboratory, attention was focused on protein identification using the bottom-up shotgun approach. Protein extracts derived from both samples were trypsinized and analyzed separately by 2-D LC and MS. In a complementary approach, the tryptic digests were analyzed directly by LC-ESI-MS/MS and gas-phase fractionation in the mass spectrometer. Taken together, both proteomic approaches in combination with a stringent evaluation process, resulted in the confident identification of 209 proteins in the human brain samples under investigation. | Keywords: | Gas-phase fractionation • HUPO Brain Proteome Project • Two-dimensional liquid chromatography • Validation | Document URI: | http://hdl.handle.net/1942/1628 | ISSN: | 1615-9853 | e-ISSN: | 1615-9861 | DOI: | 10.1002/pmic.200600080 | ISI #: | 000240875500008 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2007 |
Appears in Collections: | Research publications |
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