Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/1635
Title: The structural proteome of Pseudomonas aeruginosa bacteriophage phi KMV
Authors: Lavigne, R.
NOBEN, Jean-Paul 
Hertveldt, K.
Ceysens, P-J.
Briers, Y.
DUMONT, Debora 
Roucourt, B.
Krylov, V.
Mesyanzhinov, Vadim V.
ROBBEN, Johan 
Volckaert, G.
Issue Date: 2006
Publisher: Society for General Microbiology
Source: MICROBIOLOGY, 152. p. 529-534
Abstract: The structural proteome of {phi}KMV, a lytic bacteriophage infecting Pseudomonas aeruginosa, was analysed using two approaches. In one approach, structural proteins of the phage were fractionated by SDS-PAGE for identification by liquid chromatography-mass spectrometry (LC-MS). In a second approach, a whole-phage shotgun analysis (WSA) was applied. WSA uses trypsin digestion of whole phage particles, followed by reversed-phase HPLC and gas-phase fractionation of the complex peptide mixture prior to MS. The results yield a comprehensive view of structure-related proteins in {phi}KMV and suggest subtle structural differences from phage T7.
Keywords: COMPLETE GENOMIC SEQUENCE; TANDEM MASS-SPECTROMETRY; MEMBRANE-VESICLES; PROTEINS; IDENTIFICATION; INFECTION; VIRIONS; SP6
Document URI: http://hdl.handle.net/1942/1635
ISSN: 0026-2617
e-ISSN: 1608-3237
DOI: 10.1099/mic.0.28431-0
ISI #: 000235272400026
Category: A1
Type: Journal Contribution
Validations: ecoom 2007
Appears in Collections:Research publications

Show full item record

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.