Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/1635
Title: The structural proteome of Pseudomonas aeruginosa bacteriophage phi KMV
Authors: Lavigne, R.
NOBEN, Jean-Paul 
Hertveldt, K.
Ceysens, P-J.
Briers, Y.
DUMONT, Debora 
Roucourt, B.
Krylov, V.
Mesyanzhinov, Vadim V.
ROBBEN, Johan 
Volckaert, G.
Issue Date: 2006
Publisher: Society for General Microbiology
Source: MICROBIOLOGY, 152. p. 529-534
Abstract: The structural proteome of {phi}KMV, a lytic bacteriophage infecting Pseudomonas aeruginosa, was analysed using two approaches. In one approach, structural proteins of the phage were fractionated by SDS-PAGE for identification by liquid chromatography-mass spectrometry (LC-MS). In a second approach, a whole-phage shotgun analysis (WSA) was applied. WSA uses trypsin digestion of whole phage particles, followed by reversed-phase HPLC and gas-phase fractionation of the complex peptide mixture prior to MS. The results yield a comprehensive view of structure-related proteins in {phi}KMV and suggest subtle structural differences from phage T7.
Keywords: COMPLETE GENOMIC SEQUENCE; TANDEM MASS-SPECTROMETRY; MEMBRANE-VESICLES; PROTEINS; IDENTIFICATION; INFECTION; VIRIONS; SP6
Document URI: http://hdl.handle.net/1942/1635
ISSN: 0026-2617
e-ISSN: 1608-3237
DOI: 10.1099/mic.0.28431-0
ISI #: 000235272400026
Category: A1
Type: Journal Contribution
Validations: ecoom 2007
Appears in Collections:Research publications

Show full item record

SCOPUSTM   
Citations

58
checked on Sep 3, 2020

WEB OF SCIENCETM
Citations

59
checked on May 21, 2022

Page view(s)

18
checked on May 27, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.