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Title: | The structural proteome of Pseudomonas aeruginosa bacteriophage phi KMV | Authors: | Lavigne, R. NOBEN, Jean-Paul Hertveldt, K. Ceysens, P-J. Briers, Y. DUMONT, Debora Roucourt, B. Krylov, V. Mesyanzhinov, Vadim V. ROBBEN, Johan Volckaert, G. |
Issue Date: | 2006 | Publisher: | Society for General Microbiology | Source: | MICROBIOLOGY, 152. p. 529-534 | Abstract: | The structural proteome of {phi}KMV, a lytic bacteriophage infecting Pseudomonas aeruginosa, was analysed using two approaches. In one approach, structural proteins of the phage were fractionated by SDS-PAGE for identification by liquid chromatography-mass spectrometry (LC-MS). In a second approach, a whole-phage shotgun analysis (WSA) was applied. WSA uses trypsin digestion of whole phage particles, followed by reversed-phase HPLC and gas-phase fractionation of the complex peptide mixture prior to MS. The results yield a comprehensive view of structure-related proteins in {phi}KMV and suggest subtle structural differences from phage T7. | Keywords: | COMPLETE GENOMIC SEQUENCE; TANDEM MASS-SPECTROMETRY; MEMBRANE-VESICLES; PROTEINS; IDENTIFICATION; INFECTION; VIRIONS; SP6 | Document URI: | http://hdl.handle.net/1942/1635 | ISSN: | 0026-2617 | e-ISSN: | 1608-3237 | DOI: | 10.1099/mic.0.28431-0 | ISI #: | 000235272400026 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2007 |
Appears in Collections: | Research publications |
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