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http://hdl.handle.net/1942/16355
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DC Field | Value | Language |
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dc.contributor.author | Van den Broecke, Lynn | - |
dc.contributor.author | Vanfleteren, Jacques | - |
dc.contributor.author | Martens, Koen | - |
dc.contributor.author | SCHON, Isa | - |
dc.date.accessioned | 2014-02-19T11:39:18Z | - |
dc.date.available | 2014-02-19T11:39:18Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | BELGIAN JOURNAL OF ZOOLOGY, 143 (2), p. 106-118 | - |
dc.identifier.issn | 0777-6276 | - |
dc.identifier.uri | http://hdl.handle.net/1942/16355 | - |
dc.description.abstract | Ostracoda or mussel-shrimps are small, bivalved Crustacea. Because of their excellent fossil record and their broad variety of reproductive modes, ostracods are of great interest as a model group in ecological and evolutionary research. Here, we investigated damage and repair from one of the most important biological mutagens, namely UVB radiation, in the putative ancient asexual ostracod Darwinula stevensoni from Belgium. We applied three different methods: the Polymerase Inhibition (PI) assay, Enzyme-Linked Immuno Sorbent Assay (ELISA) and dot blot. All three techniques were unsuccessful in quantifying UVB damage in D. stevensoni. Previous experiments have revealed that the valves of D. stevensoni provide an average UVB protection of approximate 60%. Thus, UVB damage could be too little to make quantitative experiments work. Additionally, variation between individual ostracods due to season and age most likely contributed further to the failure of the three used experimental approaches to quantify damage. In a second experiment, we investigated the influence of temperature on survival of D. stevensoni during UVB exposure. The estimated relative lethal UVB dose at 4 C was with 50 kJ/m(2), significantly lower than at room temperature, with 130 kJ/m(2). This could either indicate lack of adaptation to low temperatures and/or the presence of metabolic processes active at room temperature protecting against UVB damage in D. stevensoni. The latter possibility could also explain why the estimated relative lethal UVB dose of D. stevensoni is similar to that of other non-marine ostracods where valves provide around 80% protection, despite the valves of D. stevensoni providing less protection. If such metabolic processes can repair UVB damage quickly, this may provide an alternative explanation why we could not quantify UVB damage in D. stevensoni. | - |
dc.description.sponsorship | We thank Claudine Devries-Duchene and Zohra Elouaazizi for technical support. Prof Dirk Verschuren (Ugent) is acknowledged for the use of his cold chamber for the temperaturedependent experiment. This research was funded by the Belgian Federal Science Policy (Belspo WI/36/103 and MO/36/015). | - |
dc.language.iso | en | - |
dc.subject.other | Darwinula stevensoni; ancient asexual; DNA repair | - |
dc.title | Hurdles in investigating UVB damage in the putative ancient asexual Darwinula stevensoni (Ostracoda, Crustacea) | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 118 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 106 | - |
dc.identifier.volume | 143 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Schon, I (reprint author), Royal Belgian Inst Nat Sci, Vautierstr 29, B-1000 Brussels, Belgium, isa.schoen@naturalsciences.be | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.isi | 000329549800002 | - |
item.fullcitation | Van den Broecke, Lynn; Vanfleteren, Jacques; Martens, Koen & SCHON, Isa (2013) Hurdles in investigating UVB damage in the putative ancient asexual Darwinula stevensoni (Ostracoda, Crustacea). In: BELGIAN JOURNAL OF ZOOLOGY, 143 (2), p. 106-118. | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2015 | - |
item.accessRights | Restricted Access | - |
item.contributor | Van den Broecke, Lynn | - |
item.contributor | Vanfleteren, Jacques | - |
item.contributor | Martens, Koen | - |
item.contributor | SCHON, Isa | - |
crisitem.journal.issn | 0777-6276 | - |
crisitem.journal.eissn | 2295-0451 | - |
Appears in Collections: | Research publications |
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BJZ_143_2_Vandenbroecke.pdf Restricted Access | Published version | 598.86 kB | Adobe PDF | View/Open Request a copy |
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