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http://hdl.handle.net/1942/16388
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DC Field | Value | Language |
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dc.contributor.author | TA, Duy Tien | - |
dc.contributor.author | STEEN REDEKER, Erik | - |
dc.contributor.author | GUEDENS, Wanda | - |
dc.contributor.author | ADRIAENSENS, Peter | - |
dc.date.accessioned | 2014-03-05T13:09:56Z | - |
dc.date.available | 2014-03-05T13:09:56Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | 12th Chemistry Conference for Young Scientists, Blankenberge, 27-29/02/2014 | - |
dc.identifier.uri | http://hdl.handle.net/1942/16388 | - |
dc.description.abstract | Specificity, sensitivity and reproducibility are critical issues to be improved regarding a homogeneously oriented protein coupling to surfaces for the development of next generation functional bio-materials such as biosensors. In the present study, we describe the development of a robust enzymatic strategy to accomplish all these requirements using the recombinant sortase A. This transpeptidase, derived from the bacterium Staphylococcus aureus, recognizes the conserved LPETG motif in a protein sequence and catalyzes the transpeptidation of an oligoglycine to this protein target. Particularly, the LPETG motif is engineered at the C-terminal region of the variable domain of the single-domain heavy chain antibody (or nanobody) targeting the Vascular Cell Adhesion Molecule 1 (VCAM1). The sortase A-catalyzed transpeptidation is subsequently performed to ligate the engineered nanobodies to various oligoglycine-containing co-substrates including labeling molecules, ‘clickable’ functionalities for further ‘click’ chemistry-mediated coupling, or material surfaces. | - |
dc.description.sponsorship | FWO | - |
dc.language.iso | en | - |
dc.subject.other | nanobody; sortase A; surface coupling; VCAM1 | - |
dc.title | Sortase A‐mediated protein ligation for site‐specific ‘click’ functionalization and oriented coupling of nanobodies to biosensor material surfaces or contrast probes | - |
dc.type | Conference Material | - |
local.bibliographicCitation.conferencedate | 27-29/02/2014 | - |
local.bibliographicCitation.conferencename | 12th Chemistry Conference for Young Scientists | - |
local.bibliographicCitation.conferenceplace | Blankenberge | - |
local.bibliographicCitation.jcat | C2 | - |
local.type.refereed | Refereed | - |
local.type.specified | Poster | - |
item.fulltext | With Fulltext | - |
item.fullcitation | TA, Duy Tien; STEEN REDEKER, Erik; GUEDENS, Wanda & ADRIAENSENS, Peter (2014) Sortase A‐mediated protein ligation for site‐specific ‘click’ functionalization and oriented coupling of nanobodies to biosensor material surfaces or contrast probes. In: 12th Chemistry Conference for Young Scientists, Blankenberge, 27-29/02/2014. | - |
item.contributor | TA, Duy Tien | - |
item.contributor | STEEN REDEKER, Erik | - |
item.contributor | GUEDENS, Wanda | - |
item.contributor | ADRIAENSENS, Peter | - |
item.accessRights | Open Access | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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Abstract_Tien_KVCV-final.pdf | Conference material | 644.26 kB | Adobe PDF | View/Open |
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