Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/16431
Title: | Mesenchymal stem cell secreted platelet derived growth factor exerts a pro-migratory effect on resident Cardiac Atrial appendage Stem Cells | Authors: | WINDMOLDERS, Severina De Boeck, Astrid KONINCKX, Remco Daniëls, Annick De Weyer, Olivier Bracke, Marc HENDRIKX, Marc HENSEN, Karen RUMMENS, Jean-Luc |
Issue Date: | 2014 | Source: | JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 66, p. 177-188 | Abstract: | Mesenchymal stem cells (MSCs) modulate cardiac healing after myocardial injury through the release of paracrine factors, but the exact mechanisms are still unknown. One possible mechanism is through mobilization of endogenous cardiac stemcells (CSCs). This study aimed to test the pro-migratory effect ofMSC conditionedmedium (MSC-CM) on endogenous CSCs from human cardiac tissue. By using a three-dimensional collagen assay, we found that MSC-CM improved migration of cells from human cardiac tissue. Cell counts, perimeter and area measurements were utilized to quantify migration effects. To examine whether resident stem cells were among the migrating cells, specific stem cell properties were investigated. The migrating cells displayed strong similarities with resident Cardiac Atrial appendage Stem Cells (CASCs), including a clonogenic potential of~21.5% and expression of pluripotency associated genes like Oct-4, Nanog, c-Myc and Klf-4. Similar to CASCs,migrating cells demonstrated high aldehyde dehydrogenase activity and were able to differentiate towards cardiomyocytes. Receptor tyrosine kinase analysis and collagen assays performed with recombinant platelet derived growth factor (PDGF)-AA and ImatinibMesylate, a PDGF receptor inhibitor, suggested a role for the PDGFAA/PDGF receptor α axis in enhancing the migration process of CASCs. In conclusion, our findings demonstrate that factors present in MSC-CM improve migration of resident stem cells from human cardiac tissue. These data open doors towards future therapies inwhichMSC secreted factors, like PDGF-AA, can be utilized to enhance the recruitment of CASCs towards the site of myocardial injury. | Notes: | Rummens, JL (reprint author),Jessa Hosp, Lab Expt Hematol, Campus Virga Jesse,Stadsomvaart 11, B-3500 Hasselt, Belgium severina.windmolders@uhasselt.be; adeboeck@ucalgary.ca; remco.koninckx@jessazh.be; annick.daniels@jessazh.be; olivier.dewever@ugent.be; marcl.bracke@ugent.be; marc.hendrilor@jessazh.be; karen.hensen@jessazh.be; jean-luc.rummens@jessazh.be | Keywords: | myocardial infarction; mesenchymal stem cells; conditioned medium; cardiac stem cells; migration; platelet derived growth factor | Document URI: | http://hdl.handle.net/1942/16431 | ISSN: | 0022-2828 | e-ISSN: | 1095-8584 | DOI: | 10.1016/j.yjmcc.2013.11.016 | ISI #: | 000330080700019 | Rights: | © 2013 Elsevier Ltd. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2015 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
windmolders 1.pdf Restricted Access | Published version | 2.19 MB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
29
checked on Sep 7, 2020
WEB OF SCIENCETM
Citations
38
checked on Oct 13, 2024
Page view(s)
190
checked on Sep 7, 2022
Download(s)
158
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.