Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16444
Title: Problematic barcoding in flatworms: A case-study on monogeneans and rhabdocoels (Platyhelminthes)
Authors: VANHOVE, Maarten 
TESSENS, Bart 
Schoelinck, Charlotte
Jondelius, Ulf
Littlewood, D. Tim J.
Huyse, Tine
ARTOIS, Tom 
Issue Date: 2013
Source: ZOOKEYS (365), p. 355-379
Abstract: Some taxonomic groups are less amenable to mitochondrial DNA barcoding than others. Due to the paucity of molecular information of understudied groups and the huge molecular diversity within flatworms, primer design has been hampered. Indeed, all attempts to develop universal flatworm-specific COI markers have failed so far. We demonstrate how high molecular variability and contamination problems limit the possibilities for barcoding using standard COI-based protocols in flatworms. As a consequence, molecular identification methods often rely on other widely applicable markers. In the case of Monogenea, a very diverse group of platyhelminth parasites, and Rhabdocoela, representing one-fourth of all free-living flatworm taxa, this has led to a relatively high availability of nuclear ITS and 18S/28S rDNA sequences on GenBank. In a comparison of the effectiveness in species assignment we conclude that mitochondrial and nuclear ribosomal markers perform equally well. In case intraspecific information is needed, rDNA sequences can guide the selection of the appropriate (i.e. taxon-specific) COI primers if available.
Notes: Vanhove, MPM (reprint author), Masaryk Univ, Fac Sci, Dept Bot & Zool, CS-61137 Brno, Czech Republic. maarten.vanhove@bio.kuleuven.be
Keywords: mitochondrial DNA; Monogenea; primer design; ribosomal DNA; Rhabdocoela; turbellarians
Document URI: http://hdl.handle.net/1942/16444
ISSN: 1313-2989
e-ISSN: 1313-2970
DOI: 10.3897/zookeys.365.5776
ISI #: 000329206600021
Category: A1
Type: Journal Contribution
Validations: ecoom 2015
Appears in Collections:Research publications

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