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http://hdl.handle.net/1942/16502
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DC Field | Value | Language |
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dc.contributor.author | Martins, Luisa Louro | - |
dc.contributor.author | Mourato, Miguel Pedro | - |
dc.contributor.author | Baptista, Sergio | - |
dc.contributor.author | AMARAL DOS REIS, Rafaela | - |
dc.contributor.author | Carvalheiro, Florbela | - |
dc.contributor.author | Almeida, André M. | - |
dc.contributor.author | Fevereiro, Pedro | - |
dc.contributor.author | CUYPERS, Ann | - |
dc.date.accessioned | 2014-03-26T09:09:49Z | - |
dc.date.available | 2014-03-26T09:09:49Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | ACTA PHYSIOLOGIAE PLANTARUM, 36 (3), p. 755-765 | - |
dc.identifier.issn | 0137-5881 | - |
dc.identifier.uri | http://hdl.handle.net/1942/16502 | - |
dc.description.abstract | The response of tobacco plants genetically engineered with the AtTPS1 gene to stress induced by excess Cu and Cd was evaluated in hydroponic solution (100 and 400 μM Cu and 50 and 200 μM Cd) after a 48 h exposure. Two transgenic lines, transformed with the AtTPS1 (trehalose-6-phosphate synthase) gene from Arabidopsis, with different levels of trehalose-6-phosphate synthase expression (B5H, higher and B1F, lower), and a wild type (WT) were investigated. Protein content, antioxidative enzymes (CAT, POD, SOD, and APX), glucose, fructose, lipid peroxidation, hydrogen peroxide and Cd and Cu contents were determined in leaves. The two transgenic lines were differently influenced by Cd and Cu exposure as they induced a different antioxidant enzymatic defense response. B1F and B5H plants showed a better acclimation to Cd and excess Cu compared to WT. Furthermore B1F was more tolerant than B5H to Cd and excess Cu. B1F accumulated less Cd and Cu in leaves, probably due to a more efficient exclusion mechanism. Catalase was shown to be the most important enzyme in the antioxidative system of these plants. | - |
dc.description.sponsorship | Author AM Almeida acknowledges funding from the FCT-Fundacao para a Ciencia e a Tecnologia (Lisboa, Portugal)-in the form of grant PRAXIS XXI/BD/21270/1999 that allowed the development of the transgenic plant lines and also a research contract by the Ciencia 2007 program. This work was funded by Research Project PTDC/AGR-AAM/102821/2008-Plant responses to trace element toxicity: cellular mechanisms for detoxification and tolerance-and also by FCT. The UIQA (Unidade de Investigacao Quimica Ambiental, Research Unit Environmental Chemistry) is grant aided by the FCT. The authors acknowledge the technical assistance of Maria do Ceu Penedo in analytical determination of sugars and of Dulce Santos and Susana Araujo in the production of transgenic plants. | - |
dc.language.iso | en | - |
dc.rights | © Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2013. | - |
dc.subject.other | antioxidative enzymes; cadmium; copper; oxidative stress; tobacco | - |
dc.title | Response to oxidative stress induced by cadmium and copper in tobacco plants (Nicotiana tabacum) engineered with the trehalose-6-phosphate synthase gene (AtTPS1) | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 765 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 755 | - |
dc.identifier.volume | 36 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Martins, Luisa Louro; Mourato, Miguel Pedro; Baptista, Sergio; Reis, Rafaela] Univ Tecn Lisboa, Inst Super Agron, UIQA, P-1100 Lisbon, Portugal. [Carvalheiro, Florbela] LNEG Lab Nacl Energia & Geol, Unidade Bioenergia, Lisbon, Portugal. [Almeida, Andre M.] IICT Inst Invest Cient Trop, Lisbon, Portugal. [Almeida, Andre M.; Fevereiro, Pedro] Univ Nova Lisboa, Inst Tecnol Quim & Biol, Oeiras, Portugal. [Fevereiro, Pedro] Univ Lisbon, Fac Ciencias, Dept Biol Vegetal, Lisbon, Portugal. [Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, Diepenbeek, Belgium. | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1007/s11738-013-1453-0 | - |
dc.identifier.isi | 000331632500018 | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2015 | - |
item.accessRights | Restricted Access | - |
item.fullcitation | Martins, Luisa Louro; Mourato, Miguel Pedro; Baptista, Sergio; AMARAL DOS REIS, Rafaela; Carvalheiro, Florbela; Almeida, André M.; Fevereiro, Pedro & CUYPERS, Ann (2014) Response to oxidative stress induced by cadmium and copper in tobacco plants (Nicotiana tabacum) engineered with the trehalose-6-phosphate synthase gene (AtTPS1). In: ACTA PHYSIOLOGIAE PLANTARUM, 36 (3), p. 755-765. | - |
item.contributor | Martins, Luisa Louro | - |
item.contributor | Mourato, Miguel Pedro | - |
item.contributor | Baptista, Sergio | - |
item.contributor | AMARAL DOS REIS, Rafaela | - |
item.contributor | Carvalheiro, Florbela | - |
item.contributor | Almeida, André M. | - |
item.contributor | Fevereiro, Pedro | - |
item.contributor | CUYPERS, Ann | - |
crisitem.journal.issn | 0137-5881 | - |
crisitem.journal.eissn | 1861-1664 | - |
Appears in Collections: | Research publications |
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art%3A10.1007%2Fs11738-013-1453-0.pdf Restricted Access | 472.79 kB | Adobe PDF | View/Open Request a copy |
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