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Title: Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress
Authors: Sobrino-Plata, Juan
Meyssen, Dirk
Escobar, Carolina
Hernández, Luis E.
Issue Date: 2014
Source: PLANT AND SOIL, 377 (1-2), p. 369-381
Abstract: Background Glutathione (GSH) plays a dual role under heavy metal stress, as antioxidant metabolite and as precursor of phytochelatins (PCs). Studying the responses of the GSH metabolism to heavy metals is important to improve tolerance. Methods We studied the oxidative stress signature of three γ-glutamylcysteine synthetase (γECS) Arabidopsis thaliana allele mutants (rax1-1, cad2-1, and pad2-1), first enzymatic step in the GSH synthetic pathway, when treated with 10 μM Cd or Hg for 72 h. Results GSH concentration was lower in the mutants (45 % rax1-1; 30 % cad2-1; and 20 % pad2-1), which was also associated with inferior translocation of Cd or Hg to shoots, than in wild type Col-0. Glutathione reductase (GR) and NADPH-oxidase activities were inhibited in roots, phytotoxic effects consistently more pronounced in the mutants, particularly in pad2-1. Non-photochemical quenching augmented with exposure time to Cd or Hg in Col-0, but not so in the γECS mutants. Mercury caused severe damage in cad2-1 and pad2-1 root proteins profile; toxic effects confirmed by GR and H+-ATPase immunodetection. PCs appeared in Col-0 roots under metal stress, and surprisingly accumulated in rax1-1. γECS immunodetection revealed its overexpression in rax1-1. Conclusion A minimum amount of GSH may be required for adequate metal tolerance, where γECS expression could compensate GSH deficiency under stress.
Notes: Hernandez, LE (reprint author), Univ Autonoma Madrid, Dept Biol, Lab Plant Physiol, E-28049 Madrid, Spain,
Keywords: arabidopsis thaliana; biothiols; cadmium; γ-glutamylcysteine synthetase; glutathione; mercury; phytochelatins
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ISSN: 0032-079X
e-ISSN: 1573-5036
DOI: 10.1007/s11104-013-2006-4
ISI #: 000333614600025
Rights: © Springer Science+Business Media Dordrecht 2014.
Category: A1
Type: Journal Contribution
Validations: ecoom 2015
Appears in Collections:Research publications

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