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http://hdl.handle.net/1942/16666
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DC Field | Value | Language |
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dc.contributor.author | VANDENRYT, Thijs | - |
dc.contributor.author | Pohl, Andrea | - |
dc.contributor.author | VAN GRINSVEN, Bart | - |
dc.contributor.author | THOELEN, Ronald | - |
dc.contributor.author | DE CEUNINCK, Ward | - |
dc.contributor.author | WAGNER, Patrick | - |
dc.contributor.author | Opitz, Jörg | - |
dc.date.accessioned | 2014-04-24T09:47:55Z | - |
dc.date.available | 2014-04-24T09:47:55Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | SENSORS, 13 (11), p. 14650-14661 | - |
dc.identifier.issn | 1424-8220 | - |
dc.identifier.uri | http://hdl.handle.net/1942/16666 | - |
dc.description.abstract | In this article we describe the integration of impedance spectroscopy (EIS) and surface plasmon resonance (SPR) into one surface analytic device. A polydimethylsiloxane (PDMS) flow cell is created, matching the dimensions of a commercially available sensor chip used for SPR measurements. This flow cell allowed simultaneous measurements between an EIS and a SPR setup. After a successful integration, a proof of principle study was conducted to investigate any signs of interference between the two systems during a measurement. The flow cell was rinsed with 10 mM Tris-HCl and 1× PBS buffer in an alternating manner, while impedance and shifts of the resonance angle were monitored. After achieving a successful proof of principle, a usability test was conducted. It was assessed whether simultaneous detection occurred when: (i) Protein A is adsorbed to the gold surface of the chip; (ii) The non-occupied zone is blocked with BSA molecules and (iii) IgG1 is bound to the Protein A. The results indicate a successful merge between SPR and EIS. | - |
dc.description.sponsorship | Life Science Initiative of the Province of Limburg; Special Research Funds BOF of Hasselt University; XIOS University College; Staatsministerium fur Wissenschaft und Kunst-SMWK und EFRE; European Funds for Regional Development EFRD | - |
dc.language.iso | en | - |
dc.rights | © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/) | - |
dc.subject.other | SPR; EIS; flow-cell; lab on chip; Protein A; immunoglobulin | - |
dc.title | Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 14661 | - |
dc.identifier.issue | 11 | - |
dc.identifier.spage | 14650 | - |
dc.identifier.volume | 13 | - |
local.bibliographicCitation.jcat | A1 | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.3390/s131114650 | - |
dc.identifier.isi | 000330321100023 | - |
item.fulltext | With Fulltext | - |
item.accessRights | Open Access | - |
item.validation | ecoom 2015 | - |
item.contributor | VANDENRYT, Thijs | - |
item.contributor | Pohl, Andrea | - |
item.contributor | VAN GRINSVEN, Bart | - |
item.contributor | THOELEN, Ronald | - |
item.contributor | DE CEUNINCK, Ward | - |
item.contributor | WAGNER, Patrick | - |
item.contributor | Opitz, Jörg | - |
item.fullcitation | VANDENRYT, Thijs; Pohl, Andrea; VAN GRINSVEN, Bart; THOELEN, Ronald; DE CEUNINCK, Ward; WAGNER, Patrick & Opitz, Jörg (2013) Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions. In: SENSORS, 13 (11), p. 14650-14661. | - |
crisitem.journal.eissn | 1424-8220 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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vandenryt 1.pdf | Published version | 694.97 kB | Adobe PDF | View/Open |
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