Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/16668
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dries, Eef | - |
dc.contributor.author | BITO, Virginie | - |
dc.contributor.author | Lenaerts, Ilse | - |
dc.contributor.author | Antoons, Gudrun | - |
dc.contributor.author | Sipido, Karin R. | - |
dc.contributor.author | Macquaide, Niall | - |
dc.date.accessioned | 2014-04-24T10:10:24Z | - |
dc.date.available | 2014-04-24T10:10:24Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | CIRCULATION RESEARCH, 113 (11), p. 1242-U149 | - |
dc.identifier.issn | 0009-7330 | - |
dc.identifier.uri | http://hdl.handle.net/1942/16668 | - |
dc.description.abstract | Rationale: In ventricular myocytes of large mammals with low T-tubule density, a significant number of ryanodine receptors (RyRs) are not coupled to the sarcolemma; cardiac remodeling increases noncoupled RyRs. Objective: Our aim was to test the hypothesis that coupled and noncoupled RyRs have distinct microdomain-dependent modulation. Methods and Results: We studied single myocytes from pig left ventricle. The T-tubule network was analyzed in 3-dimension (3D) to measure distance to membrane of release sites. The rising phase of the Ca2+ transient was correlated with proximity to the membrane (confocal imaging, whole-cell voltage-clamp, K5fluo-4 as Ca2+ indicator). Ca2+ sparks after stimulation were thus identified as resulting from coupled or noncoupled RyRs. We used high-frequency stimulation as a known activator of Ca2+/calmodulin-dependent kinase II. Spark frequency increased significantly more in coupled than in noncoupled RyRs. This specific modulation of coupled RyRs was abolished by the Ca2+/calmodulin-dependent kinase II blockers autocamtide-2–related inhibitory peptide and KN-93, but not by KN-92. Colocalization of Ca2+/calmodulin-dependent kinase II and RyR was not detectably different for coupled and noncoupled sites, but the F-actin disruptor cytochalasin D prevented the specific modulation of coupled RyRs. NADPH oxidase 2 inhibition by diphenyleneiodonium or apocynin, or global reactive oxygen species scavenging, also prevented coupled RyR modulation. During stimulated Ca2+ transients, frequency-dependent increase of the rate of Ca2+ rise was seen in coupled RyR regions only and abolished by autocamtide-2–related inhibitory peptide. After myocardial infarction, selective modulation of coupled RyR was lost. Conclusions: Coupled RyRs have a distinct modulation by Ca2+/calmodulin-dependent kinase II and reactive oxygen species, dependent on an intact cytoskeleton and consistent with a local Ca2+/reactive oxygen species microdomain, and subject to modification with disease. | - |
dc.description.sponsorship | Fondation Leducq Award; Belgian Science Policy Program (Grant number P6/31); European Union (Grant number Health-F2-2009-241526 EUTrigTreat); FP7 Marie Fellowship (Grant number PIEF-GA-2009-255264); Research Foundation-Flanders (FWO) PhD fellowship | - |
dc.language.iso | en | - |
dc.rights | Copyright © 2013 American Heart Association, Inc. All rights reserved. | - |
dc.subject.other | calcium/calmodulin-dependent protein kinase type 2; myocytes, cardiac; NADPH oxidase; reactive oxygen species; ryanodine receptor calcium release channel; sarcoplasmic reticulum | - |
dc.title | Selective Modulation of Coupled Ryanodine Receptors During Microdomain Activation of Calcium/Calmodulin-Dependent Kinase II in the Dyadic Cleft | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | U149 | - |
dc.identifier.issue | 11 | - |
dc.identifier.spage | 1242 | - |
dc.identifier.volume | 113 | - |
local.bibliographicCitation.jcat | A1 | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1161/CIRCRESAHA.113.301896 | - |
dc.identifier.isi | 000330353100011 | - |
item.fullcitation | Dries, Eef; BITO, Virginie; Lenaerts, Ilse; Antoons, Gudrun; Sipido, Karin R. & Macquaide, Niall (2013) Selective Modulation of Coupled Ryanodine Receptors During Microdomain Activation of Calcium/Calmodulin-Dependent Kinase II in the Dyadic Cleft. In: CIRCULATION RESEARCH, 113 (11), p. 1242-U149. | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2015 | - |
item.contributor | Dries, Eef | - |
item.contributor | BITO, Virginie | - |
item.contributor | Lenaerts, Ilse | - |
item.contributor | Antoons, Gudrun | - |
item.contributor | Sipido, Karin R. | - |
item.contributor | Macquaide, Niall | - |
item.accessRights | Restricted Access | - |
crisitem.journal.issn | 0009-7330 | - |
crisitem.journal.eissn | 1524-4571 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
dries 1.pdf Restricted Access | Published version | 7.58 MB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
23
checked on Sep 2, 2020
WEB OF SCIENCETM
Citations
38
checked on Sep 20, 2024
Page view(s)
106
checked on Sep 7, 2022
Download(s)
86
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.