A field study on solar-induced chlorophyll fluorescence and pigment parameters along a vertical canopy gradient of four tree species in an urban environment

Abstract

To better understand the potential uses of vegetation indices based on the sun-induced upward and downward chlorophyll fluorescence at leaf and at canopy scales, a field study was carried out in the city of Valencia (Spain). Fluorescence yield (FY) indices were derived for trees at different traffic intensity locations and at three canopy heights. This allowed investigating within-tree and between-tree variations of FY indices for four tree species. Several FY indices showed a significant (p b 0.05) and important effect of tree location for the species Morus alba (white mulberry) and Phoenix canariensis (Canary Island date palm). The upward FY parameters of M. alba, and the upward to downward ratios at 687 and 741 nm for both species, were significantly related to tree location. It was found that not the total chlorophyll (Chl) content, but rather the Chl a/b ratio showed the strongest correlations with several of the indices applied. Chl a/b was lowest at the bottom level of the highest traffic intensity location for both species due to an increased Chl b, indicating a larger light harvesting complex related to Photosystem II (LHCII) as a response to limiting light. The leaf deposits from traffic observed at this sampling location possibly led to a shading effect, resulting further in an adaptive response of the photosynthetic system and subsequent difference of FY indices. This study therefore indicated the importance of the size of LHCII on the fluorescence emission, observed under different traffic generated pollution conditions.