Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16693
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dc.contributor.authorNOTELAERS, Kristof-
dc.contributor.authorRocha, Susana-
dc.contributor.authorPAESEN, Rik-
dc.contributor.authorSMISDOM, Nick-
dc.contributor.authorDE CLERCQ, Ben-
dc.contributor.authorMeier, Jochen C.-
dc.contributor.authorRIGO, Jean-Michel-
dc.contributor.authorHofkens, Johan-
dc.contributor.authorAMELOOT, Marcel-
dc.date.accessioned2014-04-25T12:24:46Z-
dc.date.available2014-04-25T12:24:46Z-
dc.date.issued2014-
dc.identifier.citationBIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1843 (3), p. 544-553-
dc.identifier.issn0167-4889-
dc.identifier.urihttp://hdl.handle.net/1942/16693-
dc.description.abstractSingle particle tracking (SPT) of transmembrane receptors in the plasma membrane often reveals heterogeneous diffusion. A thorough interpretation of the displacements requires an extensive analysis suited for discrimination of differentmotion types present in the data. Here the diffusion pattern of the homomeric α3-containing glycine receptor (GlyR) is analyzed in themembrane of HEK 293 cells. More specifically, the influence of the α3 RNA splice variants α3K and α3L on lateralmembrane diffusion of the receptor is revealed in detail. Using a combination of ensemble and local SPT analysis, free and anomalous diffusion parameters are determined. The GlyR α3 free diffusion coefficient is found to be 0.13 ± 0.01 μm2/s and both receptor variants display confined motion. The confinement probability level and residence time are significantly elevated for the α3L variant compared to the α3K variant. Furthermore, for the α3L GlyR, the presence of directed motion was also established, with a velocity matching that of saltatory vesicular transport. These findings reveal that α3 GlyRs are prone to different types of anomalous diffusion and reinforce the role of RNA splicing in determining lateral membrane trafficking.-
dc.description.sponsorshipThis work was funded by the Research Council of the UHasselt, tUL. GlyR expression constructs were obtained thanks to funding by the Helmholtz Association (VH-NG-246 to J.C.M.) and the Bundesministerium fur Bildung und Forschung BMBF (Era-Net NEURON II CIPRESS to J.C.M.). The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Program (FP7/2007-2013)/ERC Grant Agreement no 291593 FLUOROCODE), from the Flemish government in the form of long-term structural funding "Methusalem" grant METH/08/04 CASAS, from the 'Fonds voor Wetenschappelijk Onderzoek Vlaanderen' (FWO grants G.0197.11; G0484.12,) and from the Hercules Foundation (HER/08/021). S.R., J.H. and M.A. thank the Federal Science Policy of Belgium (IAP-VI/27). The support by the FWO-onderzoeksgemeenschap "Scanning and Wide Field Microscopy of (Bio)-organic Systems" is gratefully acknowledged by J.H. and M.A.-
dc.language.isoen-
dc.subject.otherglycine receptor; alpha3 subunit; RNA splicing; single particle tracking; confined motion; directed motion-
dc.titleAnalysis of alpha 3 GlyR single particle tracking in the cell membrane-
dc.typeJournal Contribution-
dc.identifier.epage553-
dc.identifier.issue3-
dc.identifier.spage544-
dc.identifier.volume1843-
local.bibliographicCitation.jcatA1-
dc.description.notesAmeloot, M (reprint author), Agoralaan Gebouw C, B-3590 Diepenbeek, Belgium. marcel.ameloot@uhasselt.be-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.identifier.doi10.1016/j.bbamcr.2013.11.019-
dc.identifier.isi000330821000006-
item.fullcitationNOTELAERS, Kristof; Rocha, Susana; PAESEN, Rik; SMISDOM, Nick; DE CLERCQ, Ben; Meier, Jochen C.; RIGO, Jean-Michel; Hofkens, Johan & AMELOOT, Marcel (2014) Analysis of alpha 3 GlyR single particle tracking in the cell membrane. In: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1843 (3), p. 544-553.-
item.fulltextWith Fulltext-
item.accessRightsOpen Access-
item.validationecoom 2015-
item.contributorHofkens, Johan-
item.contributorRocha, Susana-
item.contributorSMISDOM, Nick-
item.contributorNOTELAERS, Kristof-
item.contributorDE CLERCQ, Ben-
item.contributorRIGO, Jean-Michel-
item.contributorAMELOOT, Marcel-
item.contributorPAESEN, Rik-
item.contributorMeier, Jochen C.-
crisitem.journal.issn0167-4889-
crisitem.journal.eissn1879-2596-
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