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Title: | Zymography methods for visualizing hydrolytic enzymes | Authors: | Vandooren, Jennifer GEURTS, Nathalie Martens, Erik Van den Steen, Philippe E. OPDENAKKER, Ghislain |
Issue Date: | 2013 | Publisher: | NATURE PUBLISHING GROUP | Source: | NATURE METHODS, 10 (3), p. 211-220 | Abstract: | Zymography is a technique for studying hydrolytic enzymes on the basis of substrate degradation. It is a powerful., but often misinterpreted, tool. yielding information on potential. hydrolytic activities, enzyme forms and the locations of active enzymes. In this Review, zymography techniques are compared in terms of advantages, limitations and interpretations. With in gel zymography, enzyme forms are visualized according to their molecular weights. Proteolytic activities are localized in tissue sections with in situ zymography. In vivo zymography can pinpoint proteolytic activity to sites in an intact organism. Future development of novel. substrate probes and improvement in detection and imaging methods will increase the applicability of zymography for (reverse) degradomics studies. | Notes: | [Vandooren, Jennifer; Geurts, Nathalie; Martens, Erik; Van den Steen, Philippe E.; Opdenakker, Ghislain] Univ Louvain, KU Leuven, Rega Inst Med Res, Immunobiol Lab, Louvain, Belgium. | Keywords: | Biochemical Research Methods | Document URI: | http://hdl.handle.net/1942/16914 | ISSN: | 1548-7091 | e-ISSN: | 1548-7105 | DOI: | 10.1038/NMETH.2371 | ISI #: | 000316650800016 | Rights: | © 2013 Nature America, Inc. All rights reserved. | Category: | A1 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
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Vandooren, 2013.pdf Restricted Access | 641.62 kB | Adobe PDF | View/Open Request a copy |
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