Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16959
Title: Human dental pulp stem cells can differentiate into Schwann cells and promote and guide neurite outgrowth in an aligned tissue-engineered collagen construct in vitro
Authors: MARTENS, Wendy 
SANEN, Kathleen 
Georgiou, Melanie
STRUYS, Tom 
BRONCKAERS, Annelies 
AMELOOT, Marcel 
Phillips, James
LAMBRICHTS, Ivo 
Issue Date: 2014
Source: FASEB JOURNAL, 28 (4), p. 1634-1643
Abstract: In the present study, we evaluated the differentiation potential of human dental pulp stem cells (hDPSCs) toward Schwann cells, together with their functional capacity with regard to myelination and support of neurite outgrowth in vitro. Successful Schwann cell differentiation was confirmed at the morphological and ultrastructural level by transmission electron microscopy. Furthermore, compared to undifferentiated hDPSCs, immunocytochemistry and ELISA tests revealed increased glial marker expression and neurotrophic factor secretion of differentiated hDPSCs (d-hDPSCs), which promoted survival and neurite outgrowth in 2-dimensional dorsal root ganglia cultures. In addition, neurites were myelinated by d-hDPSCs in a 3-dimensional collagen type I hydrogel neural tissue construct. This engineered construct contained aligned columns of d-hDPSCs that supported and guided neurite outgrowth. Taken together, these findings provide the first evidence that hDPSCs are able to undergo Schwann cell differentiation and support neural outgrowth in vitro, proposing them to be good candidates for cell-based therapies as treatment for peripheral nerve injury.
Keywords: neural regeneration; nerve repair; glial cell; myelination; cellular hydrogel
Document URI: http://hdl.handle.net/1942/16959
ISSN: 0892-6638
e-ISSN: 1530-6860
DOI: 10.1096/fj.13-243980
ISI #: 000335344300012
Rights: © The Author(s).
Category: A1
Type: Journal Contribution
Validations: ecoom 2015
Appears in Collections:Research publications

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