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http://hdl.handle.net/1942/17110
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DC Field | Value | Language |
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dc.contributor.author | Cambré, A. | - |
dc.contributor.author | Zimmerman, M. | - |
dc.contributor.author | Sauer, U. | - |
dc.contributor.author | Vivijs, B. | - |
dc.contributor.author | Cenens, W. | - |
dc.contributor.author | Michiels, C.W. | - |
dc.contributor.author | Aertsen, A. | - |
dc.contributor.author | Loessner, M.J. | - |
dc.contributor.author | NOBEN, Jean-Paul | - |
dc.contributor.author | Ayala, J.A. | - |
dc.contributor.author | Lavigne, R. | - |
dc.contributor.author | Briers, Y. | - |
dc.date.accessioned | 2014-09-02T15:41:56Z | - |
dc.date.available | 2014-09-02T15:41:56Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | ENVIRONMENTAL MICROBIOLOGY, 17 (5), p. 1586-1599 | - |
dc.identifier.issn | 1462-2912 | - |
dc.identifier.uri | http://hdl.handle.net/1942/17110 | - |
dc.description.abstract | Many bacteria are able to assume a transient cell wall-deficient (or L-form) state under favorable osmotic conditions. Cell wall stress such as exposure to β-lactam antibiotics can enforce the transition to and maintenance of this state. L-forms actively proliferate and can return to the walled state upon removal of the inducing agent. We have adopted Escherichia coli as a model system for the controlled transition to and reversion from the L-form state, and have studied these dynamics with genetics, cell biology and ‘omics’ technologies. As such, a transposon mutagenesis screen underscored the requirement for the Rcs phosphorelay and colanic acid synthesis, while proteomics show only little differences between rods and L-forms. In contrast, metabolome comparison reveals the high abundance of lysophospholipids and phospholipids with unsaturated or cyclopropanized fatty acids in E. coli L-forms. This increase of membrane lipids associated with increased membrane fluidity may facilitate proliferation through bud formation. Visualization of the residual peptidoglycan with a fluorescently labeled peptidoglycan binding protein indicates de novo cell wall synthesis and a role for septal peptidoglycan synthesis during bud constriction. The DD-carboxypeptidases PBP5 and PBP6 are three- and four-fold upregulated in L-forms, indicating a specific role for regulation of crosslinking during L-form proliferation. | - |
dc.language.iso | en | - |
dc.rights | This article is protected by copyright. All rights reserved. | - |
dc.title | Metabolite profiling and peptidoglycan analysis of transient cell wall-deficient bacteria in a new Escherichia coli model system. | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 1599 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 1586 | - |
dc.identifier.volume | 17 | - |
local.format.pages | 42 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Briers, Y (reprint author), Katholieke Univ Leuven, Lab Gene Technol, Dept Biosyst, B-3001 Heverlee, Belgium. yves.briers@kuleuven.be | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1111/1462-2920.12594 | - |
dc.identifier.isi | 000353507100010 | - |
item.contributor | Cambré, A. | - |
item.contributor | Zimmerman, M. | - |
item.contributor | Sauer, U. | - |
item.contributor | Vivijs, B. | - |
item.contributor | Cenens, W. | - |
item.contributor | Michiels, C.W. | - |
item.contributor | Aertsen, A. | - |
item.contributor | Loessner, M.J. | - |
item.contributor | NOBEN, Jean-Paul | - |
item.contributor | Ayala, J.A. | - |
item.contributor | Lavigne, R. | - |
item.contributor | Briers, Y. | - |
item.validation | ecoom 2016 | - |
item.fulltext | With Fulltext | - |
item.accessRights | Open Access | - |
item.fullcitation | Cambré, A.; Zimmerman, M.; Sauer, U.; Vivijs, B.; Cenens, W.; Michiels, C.W.; Aertsen, A.; Loessner, M.J.; NOBEN, Jean-Paul; Ayala, J.A.; Lavigne, R. & Briers, Y. (2015) Metabolite profiling and peptidoglycan analysis of transient cell wall-deficient bacteria in a new Escherichia coli model system.. In: ENVIRONMENTAL MICROBIOLOGY, 17 (5), p. 1586-1599. | - |
crisitem.journal.issn | 1462-2912 | - |
crisitem.journal.eissn | 1462-2920 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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emi12594.pdf | 1.24 MB | Adobe PDF | View/Open | |
cambre 1.pdf Restricted Access | Published version | 472.33 kB | Adobe PDF | View/Open Request a copy |
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