Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/17686
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dc.contributor.authorCornelis, P.-
dc.contributor.authorVANDENRYT, Thijs-
dc.contributor.authorWACKERS, Gideon-
dc.contributor.authorKELLENS, Evelien-
dc.contributor.authorLOSADA-PEREZ, Patricia-
dc.contributor.authorTHOELEN, Ronald-
dc.contributor.authorDE CEUNINCK, Ward-
dc.contributor.authorEERSELS, Kasper-
dc.contributor.authorDRIJKONINGEN, Sien-
dc.contributor.authorHAENEN, Ken-
dc.contributor.authorPEETERS, Marloes-
dc.contributor.authorVAN GRINSVEN, Bart-
dc.contributor.authorWAGNER, Patrick-
dc.date.accessioned2014-10-24T13:06:45Z-
dc.date.available2014-10-24T13:06:45Z-
dc.date.issued2014-
dc.identifier.citationDIAMOND AND RELATED MATERIALS, 48, p. 32-36-
dc.identifier.issn0925-9635-
dc.identifier.urihttp://hdl.handle.net/1942/17686-
dc.description.abstractIn this article, we report on a label-free real-time method based on heat transfer resistivity for thermal monitoring of DNA denaturation and its potential to quantify DNA fragments with a specific sequence of interest. Probe DNA, consisting of a 36-mer fragment was covalently immobilized on a nanocrystalline diamond surface, created by chemical vapor deposition on a silicon substrate. Various concentrations of full matched 29-mer target DNA fragments were hybridized with this probe DNA. We observed that the change in heat transfer resistance upon denaturation depends on the concentration of target DNA used during the hybridization, which allowed us to determine the dose-response curve. Therefore, these results illustrate the potential of this technique to quantify the concentration of a specific DNA fragment and to quantify the hybridization efficiency to its probe. (C) 2014 Elsevier B.V. All rights reserved.-
dc.description.sponsorshipLife-Science Initiative of the Province of Limburg; Research Foundation Flanders FWO [G.0829.09, G.0B62.13 N]; EU FP7 through Marie Curie ITN 420 "MATCON" [PITN-GA-2009-238201]-
dc.language.isoen-
dc.publisherELSEVIER SCIENCE SA-
dc.rights© 2014 Elsevier B.V. All rights reserved.-
dc.subject.otherCVD diamond; heat transfer resistance; DNA quantification; hybridization efficiency analysis-
dc.subject.otherCVD diamond; Heat transfer resistance; DNA quantification; Hybridization efficiency analysis-
dc.titleHeat transfer resistance as a tool to quantify hybridization efficiency of DNA on a nanocrystalline diamond surface-
dc.typeJournal Contribution-
dc.identifier.epage36-
dc.identifier.spage32-
dc.identifier.volume48-
local.format.pages5-
local.bibliographicCitation.jcatA1-
dc.description.notes[Cornelis, P.; Vandenryt, T.; Wackers, G.; Kellens, E.; Losada-Perez, P.; Thoelen, R.; De Ceuninck, W.; Eersels, K.; Drijkoningen, S.; Haenen, K.; Peeters, M.; van Grinsven, B.; Wagner, P.] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium. [Vandenryt, T.] XIOS Univ Coll, Dept Appl Engn, B-3590 Diepenbeek, Belgium. [Kellens, E.; Thoelen, R.; De Ceuninck, W.; Peeters, M.; Wagner, P.] IMOMEC, B-3590 Diepenbeek, Belgium. [van Grinsven, B.] Maastricht Univ, Maastricht Sci Programme, NL-6200 MD Maastricht, Netherlands.-
local.publisher.placeLAUSANNE-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.identifier.doi10.1016/j.diamond.2014.06.008-
dc.identifier.isi000341900700005-
item.fulltextWith Fulltext-
item.fullcitationCornelis, P.; VANDENRYT, Thijs; WACKERS, Gideon; KELLENS, Evelien; LOSADA-PEREZ, Patricia; THOELEN, Ronald; DE CEUNINCK, Ward; EERSELS, Kasper; DRIJKONINGEN, Sien; HAENEN, Ken; PEETERS, Marloes; VAN GRINSVEN, Bart & WAGNER, Patrick (2014) Heat transfer resistance as a tool to quantify hybridization efficiency of DNA on a nanocrystalline diamond surface. In: DIAMOND AND RELATED MATERIALS, 48, p. 32-36.-
item.accessRightsRestricted Access-
item.contributorDRIJKONINGEN, Sien-
item.contributorDE CEUNINCK, Ward-
item.contributorCornelis, P.-
item.contributorVAN GRINSVEN, Bart-
item.contributorLOSADA-PEREZ, Patricia-
item.contributorEERSELS, Kasper-
item.contributorHAENEN, Ken-
item.contributorWACKERS, Gideon-
item.contributorPEETERS, Marloes-
item.contributorVANDENRYT, Thijs-
item.contributorKELLENS, Evelien-
item.contributorTHOELEN, Ronald-
item.contributorWAGNER, Patrick-
item.validationecoom 2015-
crisitem.journal.issn0925-9635-
crisitem.journal.eissn1879-0062-
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