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Title: Colorectal cancer biomarker discovery and validation using LC-MS/MS-based proteomics in blood: truth or dare?
Authors: Reumer, Ank
Maes, Evelyne
Mertens, Inge
Cho, William C. S.
Landuyt, Bart
Schoofs, Liliane
Baggerman, Geert
Issue Date: 2014
Source: EXPERT REVIEW OF PROTEOMICS, 11 (4), p. 449-463
Abstract: Globally, colorectal cancer (CRC) is the third most common malignant neoplasm. However, highly sensitive, specific, noninvasive tests that allow CRC diagnosis at an early stage are still needed. As circulatory blood reflects the physiological status of an individual and/or the disease status for several disorders, efforts have been undertaken to identify candidate diagnostic CRC markers in plasma and serum. In this review, the challenges, bottlenecks and promising properties of mass spectrometry (MS)-based proteomics in blood are discussed. More specifically, important aspects in clinical design, sample retrieval, sample preparation, and MS analysis are presented. The recent developments in targeted MS approaches in plasma or serum are highlighted as well.
Notes: [Reumer, Ank; Maes, Evelyne; Landuyt, Bart; Schoofs, Liliane] Katholieke Univ Leuven, Anim Physiol & Neurobiol Sect, BE-3000 Leuven, Belgium. [Maes, Evelyne; Mertens, Inge; Valkenborg, Dirk; Baggerman, Geert] Flemish Inst Technol Res VITO, BE-2400 Mol, Belgium. [Mertens, Inge; Valkenborg, Dirk; Baggerman, Geert] Ctr Prote CFP, BE-2020 Antwerp, Belgium. [Cho, William C. S.] Queen Elizabeth Hosp, Kowloon, Hong Kong, Peoples R China. [Valkenborg, Dirk] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, BE-3590 Diepenbeek, Belgium.
Keywords: biomarker; colorectal cancer; plasma; serum; shotgun proteomics; targeted proteomics;biomarker; colorectal cancer; plasma; serum; shotgun proteomics; targeted proteomics
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ISSN: 1478-9450
e-ISSN: 1744-8387
DOI: 10.1586/14789450.2014.905743
ISI #: 000342232500006
Category: A1
Type: Journal Contribution
Validations: ecoom 2015
Appears in Collections:Research publications

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