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dc.contributor.authorten Oever, Jaap-
dc.contributor.authorvan de Veerdonk, Frank L.-
dc.contributor.authorJoosten, Leo A. B.-
dc.contributor.authorSimon, Anna-
dc.contributor.authorvan Crevel, Reinout-
dc.contributor.authorKullberg, Bart-Jan-
dc.contributor.authorGYSSENS, Inge-
dc.contributor.authorvan der Meer, Jos W. M.-
dc.contributor.authorvan Deuren, Marcel-
dc.contributor.authorNetea, Mihai G.-
dc.identifier.citationCLINICAL AND VACCINE IMMUNOLOGY, 21 (8), p. 1061-1069-
dc.description.abstractCytokine production assays have been primarily used in research settings studying novel immunodeficiencies. We sought to determine the diagnostic value of cytokine production assays in patients with recurrent and/or severe infectious diseases (IDs) without known immunodeficiencies and unclassified noninfectious inflammatory disorders (NIIDs). We retrospectively examined cytokine production in whole-blood and peripheral blood mononuclear cell samples from 157 adult patients. A cytokine production rate of <5% of that of healthy controls was considered defective. While monocyte-derived cytokine (tumor necrosis factor alpha [TNF-alpha], interleukin-1 beta [IL-1 beta], and IL-6) production was rarely affected, 30% of all included patients had deficient production of interferon gamma (IFN-gamma), IL-17A, or IL-22. Twenty-five percent of the NIID patients displayed defective IFN-gamma production, whereas IL-17A production was generally unaffected. In the group of ID patients, defective IFN-gamma production was found in 19% and 14% of the patients with viral and bacterial infections, respectively, and in 38%, 24%, and 50% of patients with mycobacterial, mucocutaneous, and invasive fungal infections, respectively. Defective IL-17A and IL-22 production was mainly confined to ID patients with mucocutaneous fungal infections. In conclusion, cytokine production assays frequently detect defective Th1 responses in patients with mycobacterial or fungal infections, in contrast to patients with respiratory tract infections or isolated bacterial infections. Defective IL-17A and IL-22 production was primarily found in patients with fungal infections, while monocyte-derived cytokine production was unaffected. Thus, lymphocyte-derived cytokine production assays are helpful in the diagnostic workup of patients with recurrent infections and suspected immunodeficiencies and have the potential to reveal immune defects that might guide adjunctive immunomodulatory therapy.-
dc.description.sponsorshipJ.T.O. was supported by the European Regional Development Fund-Province of Gelderland project 2009-010034. F. L. V. D. V. was supported by a Veni grant, and R. V. C. and A. S. were supported by a Vidi grant from the Netherlands Organization for Scientific Research (NWO). M.G.N. was supported by an ERC consolidator grant (310372).-
dc.rightsCopyright © 2014, American Society for Microbiology. All Rights Reserved.-
dc.titleCytokine Production Assays Reveal Discriminatory Immune Defects in Adults with Recurrent Infections and Noninfectious Inflammation-
dc.typeJournal Contribution-
dc.description.notes[ten Oever, Jaap; van de Veerdonk, Frank L.; Joosten, Leo A. B.; Simon, Anna; van Crevel, Reinout; Kullberg, Bart-Jan; Gyssens, Inge C.; van der Meer, Jos W. M.; van Deuren, Marcel; Netea, Mihai G.] Radboud Univ Nijmegen, Med Ctr, Dept Internal Med, NL-6525 ED Nijmegen, Netherlands. [Gyssens, Inge C.] Canisius Wilhelmina Ziekenhuis, Dept Med Microbiol & Infect Dis, Nijmegen, Netherlands. [Gyssens, Inge C.] Hasselt Univ, Diepenbeek, Belgium.-
item.fulltextNo Fulltext-
item.validationecoom 2015-
item.contributorten Oever, Jaap-
item.contributorvan Crevel, Reinout-
item.contributorKullberg, Bart-Jan-
item.contributorNetea, Mihai G.-
item.contributorvan Deuren, Marcel-
item.contributorSimon, Anna-
item.contributorvan der Meer, Jos W. M.-
item.contributorvan de Veerdonk, Frank L.-
item.contributorGYSSENS, Inge-
item.contributorJoosten, Leo A. B.-
item.fullcitationten Oever, Jaap; van de Veerdonk, Frank L.; Joosten, Leo A. B.; Simon, Anna; van Crevel, Reinout; Kullberg, Bart-Jan; GYSSENS, Inge; van der Meer, Jos W. M.; van Deuren, Marcel & Netea, Mihai G. (2014) Cytokine Production Assays Reveal Discriminatory Immune Defects in Adults with Recurrent Infections and Noninfectious Inflammation. In: CLINICAL AND VACCINE IMMUNOLOGY, 21 (8), p. 1061-1069.-
item.accessRightsClosed Access-
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