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http://hdl.handle.net/1942/17874
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DC Field | Value | Language |
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dc.contributor.author | JANSSEN, Bram | - |
dc.contributor.author | Byun, H. M. | - |
dc.contributor.author | COX, Bianca | - |
dc.contributor.author | GYSELAERS, Wilfried | - |
dc.contributor.author | Izzi, B. | - |
dc.contributor.author | Baccarelli, Andrea A. | - |
dc.contributor.author | NAWROT, Tim | - |
dc.date.accessioned | 2014-11-25T09:50:10Z | - |
dc.date.available | 2014-11-25T09:50:10Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | PLACENTA, 35 (9), p. 665-672 | - |
dc.identifier.issn | 0143-4004 | - |
dc.identifier.uri | http://hdl.handle.net/1942/17874 | - |
dc.description.abstract | Background: Epigenetics is tissue-specific and potentially even cell-specific, but little information is available from human reproductive studies about the concordance of DNA methylation patterns in cord blood and placenta, as well as within-placenta variations. We evaluated methylation levels at promoter regions of candidate genes in biological ageing pathways (SIRT1, TP53, PPARG, PPARGC1A, and TFAM), a subtelomeric region (D4Z4) and the mitochondrial genome (MT-RNR1, D-loop). Methods: Ninety individuals were randomly chosen from the ENVIRONAGE birth cohort to evaluate methylation concordance between cord blood and placenta using highly quantitative bisulfite-PCR pyrosequencing. In a subset of nineteen individuals, a more extensive sampling scheme was performed to examine within-placenta variation. Results: The DNA methylation levels of the subtelomeric region and mitochondrial genome showed concordance between cord blood and placenta with correlation coefficients ranging from r = 0.31 to 0.43, p <= 0.005, and also between the maternal and foetal sides of placental tissue (r = 0.53 to 0.72, p <= 0.05). For the majority of targets, an agreement in methylation levels between four foetal biopsies was found (with intra-class correlation coefficients ranging from 0.16 to 0.72), indicating small within-placenta variation. Conclusions: The methylation levels of the subtelomeric region (D4Z4) and mitochondrial genome (MT-RNR1, D-loop) showed concordance between cord blood and placenta, suggesting a common epigenetic signature of these targets between tissues. Concordance was lacking between the other genes that were studied. In placental tissue, methylation patterns of most targets on the mitochondrial-telomere axis were not strongly influenced by sample location. | - |
dc.description.sponsorship | The ENVIRONAGE birth cohort is supported by grants from the European Research Council (ERC), the Flemish Scientific Fund (FWO, 1.5.158.09.N.00/G.0753.10) and BOF. This work was also supported by funding from the National Institute of Environmental Health Sciences (R01ES021733 and R01ES020268). | - |
dc.language.iso | en | - |
dc.rights | © 2014 Elsevier Ltd. All rights reserved. | - |
dc.subject.other | ageing; cord blood; concordance; DNA methylation; epigenetics; placenta; within-placenta variation | - |
dc.title | Variation of DNA methylation in candidate age-related targets on the mitochondrial-telomere axis in cord blood and placenta | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 672 | - |
dc.identifier.issue | 9 | - |
dc.identifier.spage | 665 | - |
dc.identifier.volume | 35 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Nawrot, TS (reprint author), Hasselt Univ, Ctr Environm Sci, Agoralaan Gebouw D, B-3590 Diepenbeek, Belgium.bram.janssen@uhasselt.be; tim.nawrot@uhasselt.be | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1016/j.placenta.2014.06.371 | - |
dc.identifier.isi | 000342961400001 | - |
item.fulltext | With Fulltext | - |
item.fullcitation | JANSSEN, Bram; Byun, H. M.; COX, Bianca; GYSELAERS, Wilfried; Izzi, B.; Baccarelli, Andrea A. & NAWROT, Tim (2014) Variation of DNA methylation in candidate age-related targets on the mitochondrial-telomere axis in cord blood and placenta. In: PLACENTA, 35 (9), p. 665-672. | - |
item.contributor | JANSSEN, Bram | - |
item.contributor | Byun, H. M. | - |
item.contributor | COX, Bianca | - |
item.contributor | GYSELAERS, Wilfried | - |
item.contributor | Izzi, B. | - |
item.contributor | Baccarelli, Andrea A. | - |
item.contributor | NAWROT, Tim | - |
item.validation | ecoom 2015 | - |
item.accessRights | Restricted Access | - |
crisitem.journal.issn | 0143-4004 | - |
crisitem.journal.eissn | 1532-3102 | - |
Appears in Collections: | Research publications |
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janssen 1.pdf Restricted Access | Published version | 874.28 kB | Adobe PDF | View/Open Request a copy |
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