Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/17874
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dc.contributor.authorJANSSEN, Bram-
dc.contributor.authorByun, H. M.-
dc.contributor.authorCOX, Bianca-
dc.contributor.authorGYSELAERS, Wilfried-
dc.contributor.authorIzzi, B.-
dc.contributor.authorBaccarelli, Andrea A.-
dc.contributor.authorNAWROT, Tim-
dc.date.accessioned2014-11-25T09:50:10Z-
dc.date.available2014-11-25T09:50:10Z-
dc.date.issued2014-
dc.identifier.citationPLACENTA, 35 (9), p. 665-672-
dc.identifier.issn0143-4004-
dc.identifier.urihttp://hdl.handle.net/1942/17874-
dc.description.abstractBackground: Epigenetics is tissue-specific and potentially even cell-specific, but little information is available from human reproductive studies about the concordance of DNA methylation patterns in cord blood and placenta, as well as within-placenta variations. We evaluated methylation levels at promoter regions of candidate genes in biological ageing pathways (SIRT1, TP53, PPARG, PPARGC1A, and TFAM), a subtelomeric region (D4Z4) and the mitochondrial genome (MT-RNR1, D-loop). Methods: Ninety individuals were randomly chosen from the ENVIRONAGE birth cohort to evaluate methylation concordance between cord blood and placenta using highly quantitative bisulfite-PCR pyrosequencing. In a subset of nineteen individuals, a more extensive sampling scheme was performed to examine within-placenta variation. Results: The DNA methylation levels of the subtelomeric region and mitochondrial genome showed concordance between cord blood and placenta with correlation coefficients ranging from r = 0.31 to 0.43, p <= 0.005, and also between the maternal and foetal sides of placental tissue (r = 0.53 to 0.72, p <= 0.05). For the majority of targets, an agreement in methylation levels between four foetal biopsies was found (with intra-class correlation coefficients ranging from 0.16 to 0.72), indicating small within-placenta variation. Conclusions: The methylation levels of the subtelomeric region (D4Z4) and mitochondrial genome (MT-RNR1, D-loop) showed concordance between cord blood and placenta, suggesting a common epigenetic signature of these targets between tissues. Concordance was lacking between the other genes that were studied. In placental tissue, methylation patterns of most targets on the mitochondrial-telomere axis were not strongly influenced by sample location.-
dc.description.sponsorshipThe ENVIRONAGE birth cohort is supported by grants from the European Research Council (ERC), the Flemish Scientific Fund (FWO, 1.5.158.09.N.00/G.0753.10) and BOF. This work was also supported by funding from the National Institute of Environmental Health Sciences (R01ES021733 and R01ES020268).-
dc.language.isoen-
dc.rights© 2014 Elsevier Ltd. All rights reserved.-
dc.subject.otherageing; cord blood; concordance; DNA methylation; epigenetics; placenta; within-placenta variation-
dc.titleVariation of DNA methylation in candidate age-related targets on the mitochondrial-telomere axis in cord blood and placenta-
dc.typeJournal Contribution-
dc.identifier.epage672-
dc.identifier.issue9-
dc.identifier.spage665-
dc.identifier.volume35-
local.bibliographicCitation.jcatA1-
dc.description.notesNawrot, TS (reprint author), Hasselt Univ, Ctr Environm Sci, Agoralaan Gebouw D, B-3590 Diepenbeek, Belgium.bram.janssen@uhasselt.be; tim.nawrot@uhasselt.be-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.identifier.doi10.1016/j.placenta.2014.06.371-
dc.identifier.isi000342961400001-
item.fulltextWith Fulltext-
item.fullcitationJANSSEN, Bram; Byun, H. M.; COX, Bianca; GYSELAERS, Wilfried; Izzi, B.; Baccarelli, Andrea A. & NAWROT, Tim (2014) Variation of DNA methylation in candidate age-related targets on the mitochondrial-telomere axis in cord blood and placenta. In: PLACENTA, 35 (9), p. 665-672.-
item.contributorJANSSEN, Bram-
item.contributorByun, H. M.-
item.contributorCOX, Bianca-
item.contributorGYSELAERS, Wilfried-
item.contributorIzzi, B.-
item.contributorBaccarelli, Andrea A.-
item.contributorNAWROT, Tim-
item.validationecoom 2015-
item.accessRightsRestricted Access-
crisitem.journal.issn0143-4004-
crisitem.journal.eissn1532-3102-
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