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|Title:||Photonic detection and characterization of DNA using sapphire microspheres||Authors:||MURIB, Mohammed Sharif
YEAP, Weng Siang
DE CEUNINCK, Ward
VAN GRINSVEN, Bart
Schoening, Michael J.
|Issue Date:||2014||Publisher:||SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS||Source:||JOURNAL OF BIOMEDICAL OPTICS, 19 (9)||Abstract:||A microcavity-based deoxyribonucleic acid (DNA) optical biosensor is demonstrated for the first time using synthetic sapphire for the optical cavity. Transmitted and elastic scattering intensity at 1510 nm are analyzed from a sapphire microsphere (radius 500 mu m, refractive index 1.77) on an optical fiber half coupler. The 0.43 nm angular mode spacing of the resonances correlates well with the optical size of the sapphire sphere. Probe DNA consisting of a 36-mer fragment was covalently immobilized on a sapphire microsphere and hybridized with a 29-mer target DNA. Whispering gallery modes (WGMs) were monitored before the sapphire was functionalized with DNA and after it was functionalized with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). The shift in WGMs from the surface modification with DNA was measured and correlated well with the estimated thickness of the add-on DNA layer. It is shown that ssDNA is more uniformly oriented on the sapphire surface than dsDNA. In addition, it is shown that functionalization of the sapphire spherical surface with DNA does not affect the quality factor (Q approximate to 10(4)) of the sapphire microspheres. The use of sapphire is especially interesting because this material is chemically resilient, biocompatible, and widely used for medical implants. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)||Notes:||[Murib, Mohammed Sharif; Yeap, Weng-Siang; De Ceuninck, Ward; van Grinsven, Bart; Haenen, Ken; Wagner, Patrick] Hasselt Univ, Inst Materiaalonderzoek, B-3590 Diepenbeek, Belgium. [Martens, Daan; Bienstman, Peter] Ghent Univ Informat Technol, Dept Informat Technol, Photon Res Grp, B-9000 Ghent, Belgium. [De Ceuninck, Ward; van Grinsven, Bart; Haenen, Ken; Wagner, Patrick] Interuniv Microelect Ctr Vereniging Zonder Winsto, Div Inst Materiaalonderzoek Microelekt, B-3590 Diepenbeek, Belgium. [Schoening, Michael J.] Aachen Univ Appl Sci, Inst Nano & Biotechnol, D-52428 Julich, Germany. [Michiels, Luc; Ameloot, Marcel] Hasselt Univ, BIOMED, B-3590 Diepenbeek, Belgium. [Serpenguzel, Ali] Koc Univ, Dept Phys, Microphoton Res Lab, TR-34450 Istanbul, Turkey.||Keywords:||deoxyribonucleic acid; label-free biosensor; microcavity; photonics; quality factor; sapphire microsphere; whispering gallery modes||Document URI:||http://hdl.handle.net/1942/17972||ISSN:||1083-3668||e-ISSN:||1560-2281||DOI:||10.1117/1.JBO.19.9.097006||ISI #:||000343874900030||Rights:||© 2014 Society of Photo-Optical Instrumentation Engineers.||Category:||A1||Type:||Journal Contribution||Validations:||ecoom 2015|
|Appears in Collections:||Research publications|
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