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dc.contributor.authorTHIJS, Sofie-
dc.contributor.authorVan Dillewijn, Pieter-
dc.contributor.authorSILLEN, Wouter-
dc.contributor.authorTRUYENS, Sascha-
dc.contributor.authorHOLTAPPELS, Michelle-
dc.contributor.authorD'HAEN, Jan-
dc.contributor.authorCARLEER, Robert-
dc.contributor.authorWEYENS, Nele-
dc.contributor.authorAMELOOT, Marcel-
dc.contributor.authorRamos, Juan-Luis-
dc.contributor.authorVANGRONSVELD, Jaco-
dc.identifier.citationPLANT AND SOIL, 385 (1-2), p. 15-36-
dc.description.abstractBackground and aims Numerous microorganisms have been isolated from trinitrotoluene (TNT)-contaminated soils, however TNT tends to persist, indicating that the microbial biomass or activity is insufficient for degradation. Deep-rooting trees at military sites have been found to take-up contaminants from groundwater, and the extensive root and endosphere provide ideal niches for microbial TNT-transformations. Methods We characterised the rhizosphere, root endosphere and endo-phyllosphere bacteria of Acer pseudoplatanus growing at a historically TNT-contaminated location, using 16S rRNA gene fingerprinting, bacteria isolation, oxidoreductase gene-cloning, in planta growth-promotion (PGP) tests, inoculation, plant physiology measurements and microscopy. Results Based on terminal-restriction-fragment-length-polymorphism analysis, bulk soil and rhizosphere samples were highly clustered. Proteo-and Actinobacteria dominated the rhizosphere and root endosphere, whereas Alphaproteobacteria were more abundant in shoots and Actinobacteria in leaves. We isolated multiple PGP-bacteria and cloned 5 flavin-oxidoreductases belonging to the Old Yellow Enzyme family involved in TNT-reduction from 3 Pseudomonas spp., the leaf symbiont Stenotrophomonas chelatiphaga and the root endophyte Variovorax ginsengisola. Conclusions The inoculation with a selection of these strains, consortium CAP9, which combines efficient TNT-transformation capabilities with beneficial PGP-properties, has the ability to detoxify TNT in the bent grass (Agrostis capillaris) rhizosphere, stimulate plant growth and improve plant health under TNT stress.-
dc.description.sponsorshipThis work was supported by a grant from FWO-Flanders, Belgium, the Methusalem project 08M03VGRJ and partially by grant BIO2010-17227 of the Spanish Ministry of Competitiveness and FEDER. We thank Col Peter Philipsen, Cdt Herman Van Broeck and ADM Daniel Proot from the Belgian Defence for providing the TNT-sampling site. We thank Jenny Put for her technical help with the measurements of the HPLC-samples and dr. Regina-Michaela Wittich for her assistance with the ion-pair HPLC analysis. Moreover, we thank prof. dr. Tom Artois for the use of the fluorescence microscope, Rik Paesen for his help with the confocal microscope, prof. dr. Guido Bloemberg for providing the egfp and mCherry-plasmids and dr. Jonathan Van Hamme for proofreading the manuscript.-
dc.rights© Springer International Publishing Switzerland 2014-
dc.subject.otheragrostis capillaris; endophyte; OYE, XenB flavin oxidoreductases; plant growth promotion; rhizoremediation; trinitrotoluene-
dc.subject.otherAgrostis capillaris; Endophyte; OYE; XenB flavin oxidoreductases; Plant growth promotion; Rhizoremediation; Trinitrotoluene-
dc.titleExploring the rhizospheric and endophytic bacterial communities of Acer pseudoplatanus growing on a TNT-contaminated soil: towards the development of a rhizocompetent TNT-detoxifying plant growth promoting consortium-
dc.typeJournal Contribution-
dc.description.notes[Thijs, Sofie; Sillen, Wouter; Truyens, Sascha; Holtappels, Michelle; Carleer, Robert; Weyens, Nele; Vangronsveld, Jaco] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. [Van Dillewijn, Pieter] CSIC, Estn Expt Zaidin, E-18008 Granada, Spain. [D'Haen, Jan] IMO, B-3590 Diepenbeek, Belgium. [Ameloot, Marcel] Transnat Univ Limburg, Hasselt Univ, Biomed Res Inst, Sch Life Sci, B-3590 Diepenbeek, Belgium. [Ramos, Juan-Luis] Abengoa Res, E-41014 Seville, Spain.-
item.validationecoom 2015-
item.fulltextWith Fulltext-
item.accessRightsRestricted Access-
item.fullcitationTHIJS, Sofie; Van Dillewijn, Pieter; SILLEN, Wouter; TRUYENS, Sascha; HOLTAPPELS, Michelle; D'HAEN, Jan; CARLEER, Robert; WEYENS, Nele; AMELOOT, Marcel; Ramos, Juan-Luis & VANGRONSVELD, Jaco (2014) Exploring the rhizospheric and endophytic bacterial communities of Acer pseudoplatanus growing on a TNT-contaminated soil: towards the development of a rhizocompetent TNT-detoxifying plant growth promoting consortium. In: PLANT AND SOIL, 385 (1-2), p. 15-36.-
item.contributorVANGRONSVELD, Jaco-
item.contributorTHIJS, Sofie-
item.contributorCARLEER, Robert-
item.contributorVan Dillewijn, Pieter-
item.contributorTRUYENS, Sascha-
item.contributorSILLEN, Wouter-
item.contributorHOLTAPPELS, Michelle-
item.contributorRamos, Juan-Luis-
item.contributorWEYENS, Nele-
item.contributorAMELOOT, Marcel-
item.contributorD'HAEN, Jan-
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