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http://hdl.handle.net/1942/18644
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DC Field | Value | Language |
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dc.contributor.author | Praet, J. | - |
dc.contributor.author | SANTERMANS, Eva | - |
dc.contributor.author | Reekmans, K. | - |
dc.contributor.author | de Vocht, N. | - |
dc.contributor.author | Le Blon, D. | - |
dc.contributor.author | Hoornaert, C. | - |
dc.contributor.author | Daans, J. | - |
dc.contributor.author | Goossens, H. | - |
dc.contributor.author | Berneman, Z. | - |
dc.contributor.author | HENS, Niel | - |
dc.contributor.author | Van der Linden, A. | - |
dc.contributor.author | Ponsaerts, P. | - |
dc.date.accessioned | 2015-04-09T14:08:11Z | - |
dc.date.available | 2015-04-09T14:08:11Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Christ, Bruno; Oerlecke, Jana; Stock, Peggy (Ed.). Animal models for stem cell therapy, p. 265-283 | - |
dc.identifier.isbn | 978-1-4939-1453-1 | - |
dc.identifier.issn | 1064-3745 | - |
dc.identifier.uri | http://hdl.handle.net/1942/18644 | - |
dc.description.abstract | Preclinical animal studies involving intracerebral (stem) cell grafting are gaining popularity in many laboratories due to the reported beneficial effects of cell grafting on various diseases or traumata of the central nervous system (CNS). In this chapter, we describe a histological workflow to characterize and quantify cellular events following neural and fibroblast(-like) stem cell grafting in healthy and demyelinated CNS tissue. First, we provide standardized protocols to isolate and culture eGFP+ neural and fibroblast(-like) stem cells from embryonic mouse tissue. Second, we describe flow cytometric procedures to determine cell viability, eGFP transgene expression, and the expression of different stem cell lineage markers. Third, we explain how to induce reproducible demyelination in the CNS of mice by means of cuprizone administration, a validated mouse model for human multiple sclerosis. Fourth, the technical procedures for cell grafting in the CNS are explained in detail. Finally, an optimized and validated workflow for the quantitative histological analysis of cell graft survival and endogenous astroglial and microglial responses is provided. | - |
dc.language.iso | en | - |
dc.publisher | Springer Science + Business Media | - |
dc.relation.ispartofseries | Methods in Molecular Biology | - |
dc.rights | © Springer Science+Business Media New York 2014 | - |
dc.subject.other | neural stem cells; mouse embryonic fibroblasts; cell grafting; cuprizone; quantitative histology; inflammation | - |
dc.title | Histological characterization and quantification of cellular events following neural and fibroblast(-like) stem cell grafting in healty and demyelinated CNS tissue | - |
dc.type | Book Section | - |
local.bibliographicCitation.authors | Christ, Bruno | - |
local.bibliographicCitation.authors | Oerlecke, Jana | - |
local.bibliographicCitation.authors | Stock, Peggy | - |
dc.identifier.epage | 283 | - |
dc.identifier.spage | 265 | - |
dc.identifier.volume | 1213 | - |
local.bibliographicCitation.jcat | B2 | - |
dc.description.notes | [Praet, Jelle; Reekmans, Kristien; de Vocht, Nathalie; Le Blon, Debbie; Hoornaert, Chloe; Daans, Jasmijn; Berneman, Zwi; Ponsaerts, Peter] Univ Antwerp, Lab Expt Hematol, B-2020 Antwerp, Belgium. [Praet, Jelle; Santermans, Eva; Reekmans, Kristien; de Vocht, Nathalie; Le Blon, Debbie; Hoornaert, Chloe; Daans, Jasmijn; Goossens, Herman; Berneman, Zwi; Hens, Niel; Ponsaerts, Peter] Univ Antwerp, Vaccine & Infect Dis Inst Vaxinfectio, B-2020 Antwerp, Belgium. [Praet, Jelle; de Vocht, Nathalie; Van der Linden, Annemie] Univ Antwerp, Bioimaging Lab, B-2020 Antwerp, Belgium. [Santermans, Eva; Hens, Niel] Hasselt Univ, Ctr Stat, I Biostat, Hasselt, Belgium. [Hens, Niel] Univ Antwerp, Chermid, B-2020 Antwerp, Belgium. | - |
local.publisher.place | New York | - |
local.type.refereed | Refereed | - |
local.type.specified | Book Section | - |
local.relation.ispartofseriesnr | 1213 | - |
dc.identifier.doi | 10.1007/978-1-4939-1453-1_22 | - |
dc.identifier.isi | 000342900700023 | - |
local.bibliographicCitation.btitle | Animal models for stem cell therapy | - |
item.contributor | Praet, J. | - |
item.contributor | SANTERMANS, Eva | - |
item.contributor | Reekmans, K. | - |
item.contributor | de Vocht, N. | - |
item.contributor | Le Blon, D. | - |
item.contributor | Hoornaert, C. | - |
item.contributor | Daans, J. | - |
item.contributor | Goossens, H. | - |
item.contributor | Berneman, Z. | - |
item.contributor | HENS, Niel | - |
item.contributor | Van der Linden, A. | - |
item.contributor | Ponsaerts, P. | - |
item.accessRights | Restricted Access | - |
item.fullcitation | Praet, J.; SANTERMANS, Eva; Reekmans, K.; de Vocht, N.; Le Blon, D.; Hoornaert, C.; Daans, J.; Goossens, H.; Berneman, Z.; HENS, Niel; Van der Linden, A. & Ponsaerts, P. (2014) Histological characterization and quantification of cellular events following neural and fibroblast(-like) stem cell grafting in healty and demyelinated CNS tissue. In: Christ, Bruno; Oerlecke, Jana; Stock, Peggy (Ed.). Animal models for stem cell therapy, p. 265-283. | - |
item.fulltext | With Fulltext | - |
Appears in Collections: | Research publications |
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2014 Praet - Histological Characterization and Quantifi cation of Cellular Events Following Neural and Fibroblast(-Like) Stem Cell grafting in healthy and demyelinated CNS t.PDF Restricted Access | 1.2 MB | Adobe PDF | View/Open Request a copy |
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