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http://hdl.handle.net/1942/18712
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DC Field | Value | Language |
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dc.contributor.author | Bruneel, Bart | - |
dc.contributor.author | Mathae, Markus | - |
dc.contributor.author | PAESEN, Rik | - |
dc.contributor.author | AMELOOT, Marcel | - |
dc.contributor.author | Weninger, Wolfgang J. | - |
dc.contributor.author | Huysseune, Ann | - |
dc.date.accessioned | 2015-04-14T10:38:54Z | - |
dc.date.available | 2015-04-14T10:38:54Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | ZEBRAFISH, 12 (1), p. 1-10 | - |
dc.identifier.issn | 1545-8547 | - |
dc.identifier.uri | http://hdl.handle.net/1942/18712 | - |
dc.description.abstract | The zebrafish, a model organism for which a plethora of molecular and genetic techniques exists, has a lifelong replacing dentition of 22 pharyngeal teeth. This is in contrast to the mouse, which is the key organism in dental research but whose teeth are never replaced. Employing the zebrafish as the main organism to elucidate the mechanisms of continuous tooth replacement, however, poses at least one major problem, related to the fact that all teeth are located deep inside the body. Investigating tooth replacement thus relies on conventional histological methods, which are often laborious, time-consuming and can cause tissue deformations. In this review, we investigate the advantages and limitations of adapting current visualization techniques to dental research in zebrafish. We discuss techniques for fast sectioning, such as vibratome sectioning and high-resolution episcopic microscopy, and methods for in toto visualization, such as Alizarin red staining, micro-computed tomography, and optical projection tomography. Techniques for in vivo imaging, such as two-photon excitation fluorescence and second harmonic generation microscopy, are also covered. Finally, the possibilities of light sheet microscopy are addressed. | - |
dc.description.sponsorship | B.B. is supported by FWO-grant FWO12/ASP/031. The Center for X-ray Tomography of Ghent University and Prof. Dr. D. Adriaens are kindly thanked for their permission to use the micro-CT figure. Charlotte Gistelinck is kindly thanked for her permission to use the in vivo Alizarin red figure. M.A. acknowledges the Province of Limburg (Belgium) for the financial support within the tUL IMPULS FASE II program allowing for upgrading the laser source used in the SHG setup. M.M. would like to thank Lukas Reissig for his helpful comments. | - |
dc.language.iso | en | - |
dc.publisher | MARY ANN LIEBERT, INC | - |
dc.title | Imaging the Zebrafish Dentition: From Traditional Approaches to Emerging Technologies | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 10 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 1 | - |
dc.identifier.volume | 12 | - |
local.format.pages | 10 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Bruneel, B (reprint author), Univ Ghent, Proeftuinstr 86 N1, B-9000 Ghent, Belgium. bart.bruneel@ugent.be | - |
local.publisher.place | NEW ROCHELLE | - |
local.type.refereed | Refereed | - |
local.type.specified | Review | - |
dc.identifier.doi | 10.1089/zeb.2014.0980 | - |
dc.identifier.isi | 000348323600001 | - |
item.contributor | Bruneel, Bart | - |
item.contributor | Mathae, Markus | - |
item.contributor | PAESEN, Rik | - |
item.contributor | AMELOOT, Marcel | - |
item.contributor | Weninger, Wolfgang J. | - |
item.contributor | Huysseune, Ann | - |
item.fulltext | No Fulltext | - |
item.validation | ecoom 2016 | - |
item.fullcitation | Bruneel, Bart; Mathae, Markus; PAESEN, Rik; AMELOOT, Marcel; Weninger, Wolfgang J. & Huysseune, Ann (2015) Imaging the Zebrafish Dentition: From Traditional Approaches to Emerging Technologies. In: ZEBRAFISH, 12 (1), p. 1-10. | - |
item.accessRights | Closed Access | - |
crisitem.journal.issn | 1545-8547 | - |
crisitem.journal.eissn | 1557-8542 | - |
Appears in Collections: | Research publications |
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