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http://hdl.handle.net/1942/18761
Title: | Reduced graphene oxide-based sensing platform for electric cell-substrate impedance sensing | Authors: | LANCHE RADILLA, Ruben Pachauri, Vivek Koppenhoefer, Dieter WAGNER, Patrick Ingebrandt, Sven |
Issue Date: | 2014 | Publisher: | WILEY-V C H VERLAG GMBH | Source: | PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 211 (6), p. 1404-1409 | Abstract: | Graphene, with its unique electrical properties and biocompatibility, has become a material of choice for the development of biosensor platforms. In this study, a microelectrode array based on reduced graphene oxide (rGO) was constructed and used as a platform for electrical monitoring of cell-substrate adhesion. The rGO-based sensor arrays were designed in order to facilitate sensor pads comparable to the size of individual cells. The sensor chips were fabricated in a scalable manner via site-specific immobilization of graphene oxide flakes onto microelectrode pairs followed by reduction to rGO. The sensor chips were mounted on a measurement platform equipped with a fluidic cell. Electrical characteristics were recorded and field-effect behavior was confirmed. Sensors reacted to changes of pH value in the solution. Finally, as a proof-of-concept, the graphene oxide-based sensing platform was used for electrical cell-substrate impedance sensing of individual HEK293 cells in culture. Schematic view of the rGO-based sensor chip for electrical cell-substrate adhesion assays. (C) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim | Notes: | [Lanche, Ruben; Pachauri, Vivek; Koppenhoefer, Dieter; Ingebrandt, Sven] Univ Appl Sci Kaiserslautern, Dept Informat & Microsyst Technol, D-66482 Zweibrucken, Germany. [Wagner, Patrick] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium. | Keywords: | biosensors; dielectrophoresis; electric cell-substrate impedance sensing; graphene oxide;biosensors; dielectrophoresis; electric cell–substrate impedance sensing; graphene oxide | Document URI: | http://hdl.handle.net/1942/18761 | ISSN: | 1862-6300 | e-ISSN: | 1862-6319 | DOI: | 10.1002/pssa.201330522 | ISI #: | 000337759000030 | Rights: | © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2015 |
Appears in Collections: | Research publications |
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