Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/18993
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dc.contributor.authorSmolders, Katrien-
dc.contributor.authorLombaert, Nathalie-
dc.contributor.authorVALKENBORG, Dirk-
dc.contributor.authorBaggerman, Geert-
dc.contributor.authorArckens, Lutgarde-
dc.date.accessioned2015-07-16T07:22:57Z-
dc.date.available2015-07-16T07:22:57Z-
dc.date.issued2015-
dc.identifier.citationSCIENTIFIC REPORTS, 5-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/1942/18993-
dc.description.abstractAdvancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of >1,600 proteins of which >60% are associated with the plasma membrane, including (G-protein coupled) receptors, ion channels and transporters, and this from mm(3)-scale tissue.-
dc.description.sponsorshipWe thank L. Geenen, K. Schildermans, E. Maes and B. Van de Plas for excellent technical assistance. We are grateful to M. Christiaens for helping with making illustrations. This work was supported by grants of the Research Council KU Leuven (GOA/12/008). Katrien Smolders is supported by a Ph.D. fellowship of the Agency for Innovation through Sience and Technology Flanders (IWT Vlaanderen, 101421).-
dc.language.isoen-
dc.publisherNATURE PUBLISHING GROUP-
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/-
dc.titleAn effective plasma membrane proteomics approach for small tissue samples-
dc.typeJournal Contribution-
dc.identifier.volume5-
local.format.pages18-
local.format.pages18-
local.bibliographicCitation.jcatA1-
dc.description.notes[Smolders, Katrien; Lombaert, Nathalie; Arckens, Lutgarde] Katholieke Univ Leuven, Dept Biol, Lab Neuroplast & Neuroprote, Leuven, Belgium. [Valkenborg, Dirk; Baggerman, Geert] Vlaamse Instelling Technol Onderzoek, Unit Environm Risk & Hlth, B-2400 Mol, Belgium. [Valkenborg, Dirk; Baggerman, Geert] UAntwerp, Ctr Prote, Antwerp, Belgium. [Valkenborg, Dirk] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Hasselt, Belgium.-
local.publisher.placeLONDON-
local.type.refereedRefereed-
local.type.specifiedArticle-
dc.identifier.doi10.1038/srep10917-
dc.identifier.isi000355865400001-
item.fullcitationSmolders, Katrien; Lombaert, Nathalie; VALKENBORG, Dirk; Baggerman, Geert & Arckens, Lutgarde (2015) An effective plasma membrane proteomics approach for small tissue samples. In: SCIENTIFIC REPORTS, 5.-
item.fulltextWith Fulltext-
item.accessRightsOpen Access-
item.contributorSmolders, Katrien-
item.contributorLombaert, Nathalie-
item.contributorVALKENBORG, Dirk-
item.contributorBaggerman, Geert-
item.contributorArckens, Lutgarde-
item.validationecoom 2016-
crisitem.journal.issn2045-2322-
crisitem.journal.eissn2045-2322-
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