Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/19191
Title: Oxidative stress responses induced by uranium exposure at low pH in leaves of Arabidopsis thaliana plants
Authors: SAENEN, Eline 
HOREMANS, Nele 
VANHOUDT, Nathalie 
VANDENHOVE, Hildegarde 
BIERMANS, Geert 
van Hees, M.
Wannijn, J.
VANGRONSVELD, Jaco 
CUYPERS, Ann 
Issue Date: 2015
Source: JOURNAL OF ENVIRONMENTAL RADIOACTIVITY, 150, p. 36-43
Abstract: Anthropogenic activities have led to a widespread uranium (U) contamination in many countries. The toxic effects of U at the cellular level have mainly been investigated at a pH around 5.5, the optimal pH for hydroponically grown plants. However, since the speciation of U, and hence its toxicity, is strongly dependent on environmental factors such as the pH, it is important to investigate the effects of U at different environmentally relevant pH levels. Although U is poorly translocated from the roots to the shoots, resulting in a low U concentration in the leaves, it has been demonstrated that toxic effects in the leaves were already visible after 1 day exposure at pH 5.5, although only when exposed to relatively high U concentrations (100 mM). Therefore, the present study aimed to analyse the effects of different U concentrations (ranging from 0 to 100 mM) at pH 4.5 in leaves of Arabidopsis thaliana plants. Results indicate that U induces early senescence in A. thaliana leaves as was suggested by a decreased expression of CAT2 accompanied by an induction of CAT3 expression, a decreased CAT capacity and an increased lipid peroxidation. In addition, miRNA398b/c is involved in the regulation of the SOD response in the leaves. As such, an increased MIR398b/c expression was observed leading to a decreased transcript level of CSD1/ 2. Finally, the biosynthesis of ascorbate was induced after U exposure. This can point towards an important role for this metabolite in the scavenging of reactive oxygen species under U stress.
Notes: Saenen, E (reprint author), Boeretang 200, B-2400 Mol, Belgium. eline.saenen@sckcen.be; nele.horemans@sckcen.be; nathalie.vanhoudt@sckcen.be; hildegarde.vandenhove@sckcen.be; geert.biermans@FANC.FGOV.BE; mvhees@sckcen.be; jwannijg@sckcen.be; jaco.vangronsveld@uhasselt.be; ann.cuypers@uhasselt.be
Keywords: arabidopsis thaliana; uranium toxicity; pH; oxidative stress; senescence
Document URI: http://hdl.handle.net/1942/19191
ISSN: 0265-931X
e-ISSN: 1879-1700
DOI: 10.1016/j.jenvrad.2015.07.021
ISI #: 000364269200004
Rights: © 2015 Elsevier Ltd. All rights reserved.
Category: A1
Type: Journal Contribution
Validations: ecoom 2016
Appears in Collections:Research publications

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