Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/19837
Title: | Evaluation of established human IPSC-derived neurons to model neurodegenerative diseases | Authors: | Meneghello, G. Verheyen, A. Van Ingen, M. KUIJLAARS, Jacobine Tuefferd, M. Van den Wyngaert, I. Nuydens, R. |
Issue Date: | 2015 | Publisher: | PERGAMON-ELSEVIER SCIENCE LTD | Source: | NEUROSCIENCE, 301, p. 204-212 | Abstract: | Neurodegenerative diseases are difficult to study due to unavailability of human neurons. Cell culture systems and primary rodent cultures have shown to be indispensable to clarify disease mechanisms and provide insights into gene functions. Nevertheless, it is hard to translate new findings into new medicines. The discovery of human induced pluripotent stem cells (iPSC) might partially overcome this problem. Commercially available human iPSC-derived neurons, when thoroughly characterized and suitable for viral transduction, might represent a faster model for drugs screening than the time-consuming derivation and differentiation of iPSC from patient samples. In this study we show that iCell (R) neurons are primarily immature GABAergic neurons within the tested time frame. Addition of C6 glioma conditioned medium improved the bursting frequency of cells without further maturation or evidence for glutamatergic responses. Furthermore, cells were suitable for lentiviral transduction within the tested time frame. Altogether, iCell (R) neurons might be useful to model neurodegenerative diseases in which young GABAergic subtypes are affected. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved. | Notes: | [Meneghello, G.; Verheyen, A.; Van Ingen, M.; Tuefferd, M.; Van den Wyngaert, I.; Nuydens, R.] Janssen Pharmaceut NV, Div A, Janssen Res & Dev, B-2340 Beerse, Belgium. [Kuijlaars, J.] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. | Keywords: | disease modeling; neurodegeneration; hiPSC; GABAergic neuron;disease modeling; neurodegeneration; hiPSC; GABAergic neuron | Document URI: | http://hdl.handle.net/1942/19837 | ISSN: | 0306-4522 | e-ISSN: | 1873-7544 | DOI: | 10.1016/j.neuroscience.2015.05.071 | ISI #: | 000357877800018 | Rights: | © 2015 IBRO. Published by Elsevier Ltd. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2016 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
1-s2.0-S0306452215005229-main.pdf Restricted Access | 2.79 MB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
9
checked on Sep 2, 2020
WEB OF SCIENCETM
Citations
14
checked on Sep 28, 2024
Page view(s)
86
checked on Apr 17, 2023
Download(s)
58
checked on Apr 17, 2023
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.