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Title: | Proteomic Alterations in B Lymphocytes of Sensitized Mice in a Model of Chemical-Induced Asthma | Authors: | Haenen, Steven Vanoirbeek, Jeroen A. J. De Vooght, Vanessa Schoofs, Liliane Nemery, Benoit CLYNEN, Elke Hoet, Peter H. M. |
Issue Date: | 2015 | Publisher: | PUBLIC LIBRARY SCIENCE | Source: | PLOS ONE, 10 (9) | Abstract: | Introduction and Aim The role of B-lymphocytes in chemical-induced asthma is largely unknown. Recent work demonstrated that transferring B lymphocytes from toluene diisocyanate (TDI)-sensitized mice into naive mice, B cell KO mice and SCID mice, triggered an asthma-like response in these mice after a subsequent TDI-challenge. We applied two-dimensional difference gel electrophoresis (2D-DIGE) to describe the "sensitized signature" of B lymphocytes comparing TDI-sensitized mice with control mice. Results Sixteen proteins were identified that were significantly up- or down-regulated in B lymphocytes of sensitized mice. Particularly differences in the expression of cyclophilin A, cofilin 1 and zinc finger containing CCHC domain protein 11 could be correlated to the function of B lymphocytes as initiators of T lymphocyte independent asthma-like responses. Conclusion This study revealed important alterations in the proteome of sensitized B cells in a mouse model of chemical-induced asthma, which will have an important impact on the B cell function. | Notes: | [Haenen, Steven; Vanoirbeek, Jeroen A. J.; De Vooght, Vanessa; Nemery, Benoit; Hoet, Peter H. M.] Katholieke Univ Leuven, Ctr Environm & Hlth, Leuven, Belgium. [Haenen, Steven; Schoofs, Liliane] Katholieke Univ Leuven, Res Grp Funct Genom & Prote, Leuven, Belgium. [Clynen, Elke] Hasselt Univ, Biomed Res Inst BIOMED, Hasselt, Belgium. | Document URI: | http://hdl.handle.net/1942/19856 | ISSN: | 1932-6203 | e-ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0138791 | ISI #: | 000361797500109 | Rights: | © 2015 Haenen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2016 |
Appears in Collections: | Research publications |
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