Grassland species will not necessarily benefit from future elevated air temperatures: a chlorophyll fluorescence approach to study autumn physiology

Abstract

Model ecosystems were grown in 12 sunlit, climate-controlled chambers to gain insight into the effects of elevated (+3 degrees C) air temperature (T-air) on temperate grasslands. In this study, the hypothesis of delayed senescence in response to elevated T-air was tested for Rumex acetosa L. and Plantago lanceolata L. During the autumn of the first treatment year, frequent measurements were made of leaf chlorophyll a (Chla) fluorescence transients. Chl fluorescence images of individual leaves as well as digital colour images of these ecosystems were captured. Chl fluorescence variables, such as the maximum quantum yield of primary photochemistry (F-v/F-m), indicated a decreasing efficiency with time. Despite no treatment effect on F-v/F-m, other variables derived from the Chl fluorescence transients showed a strong trend towards a positive effect of a 3 degrees C temperature increase on the photosynthetic performance of R. acetosa and P. lanceolata in the first year. After mid-September, the initial positive treatment effect disappeared for R. acetosa, strongly suggesting that leaf lifespan of this species was shortened by higher T-air. One possible explanation is more intense drought stress in the elevated compared to the ambient temperature treatments. Second-year measurements were possibly too limited in time to confirm this trend. These results show that temperate grassland species may take advantage of a future increase in T-air during autumn. This will ultimately depend on the species' degree of acclimation to a temperature change and on the resistance to drought stress.