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Title: | Influence of nutrient medium composition on uranium toxicity and choice of the most sensitive growth related endpoint in Lemna minor | Authors: | HOREMANS, Nele Van Hees, May SAENEN, Eline Van Hoeck, Arne Smolders, Valerie Blust, Ronny VANDENHOVE, Hildegarde |
Issue Date: | 2016 | Publisher: | ELSEVIER SCI LTD | Source: | JOURNAL OF ENVIRONMENTAL RADIOACTIVITY, 151, p. 427-437 | Abstract: | Uranium (U) toxicity is known to be highly dependent on U speciation and bioavailability, To assess the impact of uranium on plants, a growth inhibition test was set up in the freshwater macrophyte Lemna minor. First growth media with different compositions were tested in order to find a medium fit for testing U toxicity in L. minor. Following arguments were used for medium selection: the ability to sustain L. minor growth, a high solubility of U in the medium and a high percentage of the more toxic U-species namely UO22+. Based on these selection criteria a with a low phosphate concentration of 0.5 mg L-1 and supplemented with 5 mM MES (2-(N-morpholino)ethanesulfonic acid) to ensure pH stability was chosen. This medium also showed highest U toxicity compared to the other tested media. Subsequently a full dose response curve for U was established by exposing L. minor plants to U concentrations ranging from 0.05 mu M up to 150 mu M for 7 days. Uranium was shown to adversely affect growth of L. minor in a dose dependent manner with EC10, EC30 and EC50 values ranging between 1.6 and 4.8 mu M, 7.7-16.4 mu M and 19.4-37.2 mu M U, respectively, depending on the growth endpoint. Four different growth related endpoints were tested: frond area, frond number, fresh weight and dry weight. Although differences in relative growth rates and associated ECx-values calculated on different endpoints are small (maximal twofold difference), frond area is recommended to be used to measure U-induced growth effects as it is a sensitive growth endpoint and easy to measure in vivo allowing for measurements over time. (C) 2015 Elsevier Ltd. All rights reserved. | Notes: | [Horemans, Nele; Van Hees, May; Saenen, Eline; Van Hoeck, Arne; Smolders, Valerie; Vandenhove, Hildegarde] Belgian Nucl Res Ctr SCK CEN, B-2400 Mol, Belgium. [Horemans, Nele] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. [Blust, Ronny] Univ Antwerp, Dept Biol, B-2020 Antwerp, Belgium. | Keywords: | Uranium toxicity; Uranium speciation; Lemna minor; Growth inhibition; Culture medium;uranium toxicity; uranium speciation; lemna minor; growth inhibition; culture medium | Document URI: | http://hdl.handle.net/1942/20582 | ISSN: | 0265-931X | e-ISSN: | 1879-1700 | DOI: | 10.1016/j.jenvrad.2015.06.024 | ISI #: | 000367767700009 | Rights: | © 2015 Elsevier Ltd. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | Research publications |
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