Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/20601
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lermyte, Frederik | - |
dc.contributor.author | Verschueren, Tim | - |
dc.contributor.author | Brown, Jeffery M. | - |
dc.contributor.author | Williams, Jonathan P. | - |
dc.contributor.author | VALKENBORG, Dirk | - |
dc.contributor.author | Sobott, Frank | - |
dc.date.accessioned | 2016-02-11T08:57:38Z | - |
dc.date.available | 2016-02-11T08:57:38Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | METHODS, 89, p. 22-29 | - |
dc.identifier.issn | 1046-2023 | - |
dc.identifier.uri | http://hdl.handle.net/1942/20601 | - |
dc.description.abstract | Top-down sequencing methods are becoming increasingly relevant for protein characterization, in particular electron capture (ECD) and electron transfer dissociation (ETD) which allow for extensive backbone cleavage with minimal side reactions. The ability to obtain sequence-specific fragments while maintaining aspects of the higher-order structure, as well as the position of deuterium labels in HID exchange, has attracted interest from scientists in the field of structural proteomics. Recently, ETD has also been combined with ion mobility on commercially available quadrupole/time-of-flight instruments, and this implementation paves the way to novel structural studies and investigation of the ETD process itself. In the current work, we investigate the use of ETD for fragmentation of standard peptides and proteins and provide a detailed description of the effect of the parameters controlling the time and efficiency of the reaction. We also highlight how the combination with ion mobility separation after electron transfer provides extended analytical benefits, such as assignment of fragments to a specific charge-reduced state of the precursor. (C) 2015 Elsevier Inc. All rights reserved. | - |
dc.description.sponsorship | We thank the Research Foundation - Flanders (FWO) for funding a PhD fellowship (F.L.). The Synapt G2 mass spectrometer is funded by a grant from the Hercules Foundation - Flanders. Financial support from the Flemish Institute for Technological Research (VITO) is gratefully acknowledged. | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.rights | © 2015 Elsevier Inc. All rights reserved. | - |
dc.subject.other | top-down mass spectrometry; intact protein mass spectrometry; top-down sequencing; electron transfer dissociation; ion mobility | - |
dc.subject.other | Top-down mass spectrometry; Intact protein mass spectrometry; Top-down sequencing; Electron transfer dissociation; Ion mobility | - |
dc.title | Characterization of top-down ETD in a travelling-wave ion guide | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 29 | - |
dc.identifier.spage | 22 | - |
dc.identifier.volume | 89 | - |
local.format.pages | 8 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Lermyte, Frederik; Verschueren, Tim; Sobott, Frank] Univ Antwerp, Dept Chem, Biomol & Analyt Mass Spectrometry Grp, B-2020 Antwerp, Belgium. [Lermyte, Frederik; Valkenborg, Dirk; Sobott, Frank] Univ Antwerp, UA VITO Ctr Prote, B-2020 Antwerp, Belgium. [Brown, Jeffery M.; Williams, Jonathan P.] Waters Corp, Wilmslow SK9 4AX, Cheshire, England. [Valkenborg, Dirk] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Hasselt, Belgium. [Valkenborg, Dirk] Flemish Inst Technol Res VITO, Appl Bio & Mol Syst, Mol, Belgium. | - |
local.publisher.place | SAN DIEGO | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1016/j.ymeth.2015.05.019 | - |
dc.identifier.isi | 000365062800004 | - |
item.validation | ecoom 2016 | - |
item.accessRights | Restricted Access | - |
item.fullcitation | Lermyte, Frederik; Verschueren, Tim; Brown, Jeffery M.; Williams, Jonathan P.; VALKENBORG, Dirk & Sobott, Frank (2015) Characterization of top-down ETD in a travelling-wave ion guide. In: METHODS, 89, p. 22-29. | - |
item.fulltext | With Fulltext | - |
item.contributor | Lermyte, Frederik | - |
item.contributor | Verschueren, Tim | - |
item.contributor | Brown, Jeffery M. | - |
item.contributor | Williams, Jonathan P. | - |
item.contributor | VALKENBORG, Dirk | - |
item.contributor | Sobott, Frank | - |
crisitem.journal.issn | 1046-2023 | - |
crisitem.journal.eissn | 1095-9130 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
lermyte 1.pdf Restricted Access | Published version | 2.16 MB | Adobe PDF | View/Open Request a copy |
SCOPUSTM
Citations
16
checked on Sep 2, 2020
WEB OF SCIENCETM
Citations
23
checked on May 10, 2024
Page view(s)
56
checked on Sep 7, 2022
Download(s)
48
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.