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http://hdl.handle.net/1942/20692
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DC Field | Value | Language |
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dc.contributor.author | Holvoet, Bryan | - |
dc.contributor.author | Quattrocelli, Mattia | - |
dc.contributor.author | Belderbos, Sarah | - |
dc.contributor.author | Pollaris, Lore | - |
dc.contributor.author | WOLFS, Esther | - |
dc.contributor.author | Gheysens, Olivier | - |
dc.contributor.author | Gijsbers, Rik | - |
dc.contributor.author | Vanoirbeek, Jeroen | - |
dc.contributor.author | Verfaillie, Catherine M. | - |
dc.contributor.author | Sampaolesi, Maurilio | - |
dc.contributor.author | Deroose, Christophe M. | - |
dc.date.accessioned | 2016-02-18T12:51:04Z | - |
dc.date.available | 2016-02-18T12:51:04Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | STEM CELL REPORTS, 5 (6), p. 1183-1195 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | http://hdl.handle.net/1942/20692 | - |
dc.description.abstract | Muscular dystrophies are a heterogeneous group of myopathies, characterized by muscle weakness and degeneration, without curative treatment. Mesoangioblasts (MABs) have been proposed as a potential regenerative therapy. To improve our understanding of the in vivo behavior of MABs and the effect of different immunosuppressive therapies, like cyclosporine A or co-stimulation-adhesion blockade therapy, on cell survival noninvasive cell monitoring is required. Therefore, cells were transduced with a lentiviral vector encoding firefly luciferase (Fluc) and the human sodium iodide transporter (hNIS) to allow cell monitoring via bioluminescence imaging (BLI) and small-animal positron emission tomography (PET). Non-H2 matched mMABs were injected in the femoral artery of dystrophic mice and were clearly visible via small-animal PET and BLI. Based on noninvasive imaging data, we were able to show that co-stim was clearly superior to CsA in reducing cell rejection and this was mediated via a reduction in cytotoxic Tcells and upregulation of regulatory Tcells. | - |
dc.description.sponsorship | The authors thank A. Van Santvoort and T. Buelens for their help in data acquisition and processing and H. Grosemans for her help in maintaining the animal colonies and molecular analyses. The radiopharmacy team from the Nuclear Medicine Department at UZ Leuven is acknowledged for 99mTcO<INF>4</INF>- tracer preparations. B.H. is the recipient of a research grant from the IWT-Vlaanderen. O.G. is a senior clinical investigator of the Fund for Scientific Research Flanders (1831812N). The M.S. Laboratory is supported by the Opening the Future Campaign EJJ-OPTFUT-02010 and FWO (#G060612N, #G0A8813N, and #G088715N). M.S. and C.M.V. would also like to thank Rondoufonds voor Duchenne Onderzoek for the kind donation. The C.M.V. Laboratory is supported by SBO-BRAINSTEM (#60838). We also acknowledge infrastructural funding by the InfraMouse Grant from the Hercules Foundation (ZW09-03). | - |
dc.language.iso | en | - |
dc.publisher | CELL PRESS | - |
dc.rights | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | - |
dc.title | Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 1195 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | 1183 | - |
dc.identifier.volume | 5 | - |
local.format.pages | 13 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Holvoet, Bryan; Belderbos, Sarah; Gheysens, Olivier; Deroose, Christophe M.] Katholieke Univ Leuven, Ctr Environm & Hlth, Dept Imaging & Pathol Nucl Med & Mol Imaging, B-3000 Leuven, Belgium. [Quattrocelli, Mattia; Sampaolesi, Maurilio] Katholieke Univ Leuven, Ctr Environm & Hlth, Translat Cardiomyol Lab, Dept Dev & Regenerat, B-3000 Leuven, Belgium. [Pollaris, Lore; Vanoirbeek, Jeroen] Katholieke Univ Leuven, Ctr Environm & Hlth, Dept Publ Hlth & Primary Care, B-3000 Leuven, Belgium. [Wolfs, Esther] Univ Hasselt, Histol Lab, Biomed Res Inst, Dept Morphol, B-3590 Diepenbeek, Belgium. [Gijsbers, Rik] Katholieke Univ Leuven, Stem Cell Inst Leuven, Lab Mol Virol & Gene Therapy, Dept Pharmaceut & Pharmacol Sci,Leuven Viral Vect, B-3000 Leuven, Belgium. [Vanoirbeek, Jeroen] Katholieke Univ Leuven, Stem Cell Inst Leuven, Dept Dev & Regenerat, B-3000 Leuven, Belgium. | - |
local.publisher.place | CAMBRIDGE | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1016/j.stemcr.2015.10.018 | - |
dc.identifier.isi | 000366142900022 | - |
item.fulltext | With Fulltext | - |
item.fullcitation | Holvoet, Bryan; Quattrocelli, Mattia; Belderbos, Sarah; Pollaris, Lore; WOLFS, Esther; Gheysens, Olivier; Gijsbers, Rik; Vanoirbeek, Jeroen; Verfaillie, Catherine M.; Sampaolesi, Maurilio & Deroose, Christophe M. (2015) Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice. In: STEM CELL REPORTS, 5 (6), p. 1183-1195. | - |
item.accessRights | Open Access | - |
item.contributor | Holvoet, Bryan | - |
item.contributor | Quattrocelli, Mattia | - |
item.contributor | Belderbos, Sarah | - |
item.contributor | Pollaris, Lore | - |
item.contributor | WOLFS, Esther | - |
item.contributor | Gheysens, Olivier | - |
item.contributor | Gijsbers, Rik | - |
item.contributor | Vanoirbeek, Jeroen | - |
item.contributor | Verfaillie, Catherine M. | - |
item.contributor | Sampaolesi, Maurilio | - |
item.contributor | Deroose, Christophe M. | - |
item.validation | ecoom 2017 | - |
crisitem.journal.issn | 2213-6711 | - |
crisitem.journal.eissn | 2213-6711 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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holvoet 1.pdf | Published version | 3.17 MB | Adobe PDF | View/Open |
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