Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2092
Title: Induction of oxidative stress and antioxidative mechanisms in Phaseolus vulgaris after Cd application
Authors: SMEETS, Karen 
CUYPERS, Ann 
Lambrechts, A
SEMANE, Brahim 
Hoet, P
Van Laere, A
VANGRONSVELD, Jaco 
Issue Date: 2005
Publisher: EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Source: PLANT PHYSIOLOGY AND BIOCHEMISTRY, 43(5). p. 437-444
Abstract: Oxidative stress has been shown to be of great importance in the toxicity of several metals (copper, zinc,...). In this study, the relationship of cadmium phytotoxicity and antioxidative reactions in bean (Phaseolus vulgaris L.) plants was investigated. Eleven-day-old seedlings were exposed to an environmentally realistic concentration of cadmium (2 mu M CdSO4) Several biochemical and physiological parameters were influenced even by these low concentrations. At the biochemical level, the antioxidative defence mechanism was significantly activated after 24 h of cadmium exposure. Some enzymes able of quenching reactive oxygen species (syringaldazine peroxidase, EC 1.11.1.7; guaiacol peroxidase, EC 1.11.1.7) as well as enzymes important in the reduction of NAD(P)(+) (isocitrate dehydrogenase, EC 1.1.1.42; malic enzyme, EC 1.1.1.40) were significantly elevated by cadmium exposure. Furthermore, the ascorbate-glutathione cycle appeared to be a very important mechanism against cadmium-induced oxidative stress. In leaves, significant increases of ascorbate peroxidase (EC 1.11.1.11) and glutathione reductase (EC 1.6.4.2) and significant changes in the ascorbate and glutathione pool were observed. Morphological and other biochemical parameters (lipid peroxidation) were significantly enhanced 48 h after the start of the cadmium exposure. At the end of the experiment (72 h after the start of the metal treatment), even visual effects, such as chlorosis, were observed. The present data indicate that cadmium, like other metals, induces cellular redox disequilibrium suggesting that an environmentally realistic concentration of cadmium can cause oxidative stress. (c) 2005 Elsevier SAS. All rights reserved.
Notes: Limburgs Univ Ctr, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. Lab Mol Plant Physiol, B-3001 Heverlee, Belgium. O & N, Pneumol, B-3000 Louvain, Belgium.Cuypers, A, Limburgs Univ Ctr, Ctr Environm Sci, Univ Campus, B-3590 Diepenbeek, Belgium.ann.cuypers@luc.ac.be
Keywords: antioxidative defence; cadmium toxicity; environmentally realistic metal-concentrations; oxidative stress; Phaseolus vulgaris
Document URI: http://hdl.handle.net/1942/2092
ISSN: 0981-9428
e-ISSN: 1873-2690
DOI: 10.1016/j.plaphy.2005.03.007
ISI #: 000229977900003
Category: A1
Type: Journal Contribution
Validations: ecoom 2006
Appears in Collections:Research publications

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