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Title: | High intensity training may reverse the fiber type specific decline in myogenic stem cells in multiple sclerosis patients | Authors: | Farup, Jean Dalgas, Ulrik KEYTSMAN, Charly OP 'T EIJNDE, Bert WENS, Inez |
Issue Date: | 2016 | Source: | Frontiers in Physiology, 7, (ART N° 193) | Abstract: | Multiple sclerosis (MS) is associated with loss of skeletal muscle mass and function. The myogenic stem cells (satellite cells – SCs) are instrumental to accretion of myonuclei, but remain to be investigated in MS. The present study aimed to compare the SC and myonuclei content between MS patients (n=23) and age matched healthy controls (HC, n=18). Furthermore, the effects of 12 weeks of high intensity training on SC and myonuclei content were explored in MS. Muscle biopsies were obtained from m. Vastus Lateralis at baseline (MS+HC) and following 12 weeks of training (MS only). Frozen biopsies were sectioned followed by immunohistochemical analysis for fiber type specific SCs (Pax7+), myonuclei (MN) and central nuclei content and fiber cross-sectional area (fCSA) using ATPase histochemistry. At baseline the SCs per fiber was lower in type II compared to type I fiber in both MS (119%, p<0.01) and HC (69%, p<0.05), whereas the SCs per fCSA was lower in type II fibers compared to type I only in MS (72%, p<0.05). No differences were observed in MN or central nuclei between MS and HC. Following training the type II fiber SCs per fiber and fCSA in MS patients increased by 165% (p<0.05) and 135% (p<0.05), respectively. Furthermore, the type II fiber MN content increased by 35% (p<0.05) following training. In conclusion, the SC content is lower in type II compared to type I fibers in both MS and HC. Furthermore, high intensity training was observed to selectively increase the SC and myonuclei content in type II fibers in MS patients. | Notes: | Correspondence: Dr. Inez Wens, Hasselt University, REVAL Rehabilitation Research Center, BIOMED Biomedical Research Institute, Faculty of Medicine and Life Sciences, Diepenbeek, Belgium, inez.wens@uhasselt.be | Keywords: | satellite cells; myonuclei; skeletal muscle atrophy; Multiple Sclerosis; Fibrosis | Document URI: | http://hdl.handle.net/1942/21150 | e-ISSN: | 1664-042X | DOI: | 10.3389/fphys.2016.00793 | ISI #: | 000376739000001 | Rights: | Copyright: © 2016 Farup, Dalgas, Keytsman, Eijnde and Wens. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | Research publications |
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fphys-07-00193.pdf | Published version | 4.31 MB | Adobe PDF | View/Open |
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