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http://hdl.handle.net/1942/21212
Title: | Optimization of isolation and cultivation of bacterial endophytes through addition of plant extract to nutrient media | Authors: | EEVERS, Nele GIELEN, Marijke Sanchez-Lopez, A. JASPERS, Stijn White, J.C. VANGRONSVELD, Jaco WEYENS, Nele |
Issue Date: | 2015 | Source: | Microbial Biotechnology, 8 (4), p. 707-715 | Abstract: | Many endophytes have beneficial effects on plants and can be exploited in biotechnological applications. Studies hypothesize that only 0.001–1% of all plantassociated bacteria are cultivable. Moreover, even after successful isolations, many endophytic bacteria often show reduced regrowth capacity. This research aimed to optimize isolation processes and culturing these bacteria afterwards. We compared several minimal and complex media in a screening. Beside the media themselves, two gelling agents and adding plant extract to media were investigated to enhance the number and diversity of endophytes as well as the growth capacity when regrown after isolation. In this work, 869 medium delivered the highest numbers of cultivable bacteria, as well as the highest diversity. When comparing gelling agents, no differences were observed in the numbers of bacteria. Adding plant extract to the media lead to a slight increase in diversity. However, when adding plant extract to improve the regrowth capacity, sharp increases of viable bacteria occurred in both rich and minimal media. | Document URI: | http://hdl.handle.net/1942/21212 | ISSN: | 1751-7907 | DOI: | 10.1111/1751-7915.12291 | ISI #: | 000356370600012 | Rights: | © 2015 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2016 |
Appears in Collections: | Research publications |
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Eevers_et_al-2015-Microbial_Biotechnology.pdf | Published version | 793.53 kB | Adobe PDF | View/Open |
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