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Title: | Protein profiling of 3T3-L1 adipocyte differentiation and (tumor necrosis factor alpha-mediated) starvation | Authors: | RENES, J. Bouwman, F NOBEN, Jean-Paul EVELO, Chris ROBBEN, Johan MARIMAN, Edwin |
Issue Date: | 2005 | Publisher: | BIRKHAUSER VERLAG AG | Source: | CELLULAR AND MOLECULAR LIFE SCIENCES, 62(4). p. 492-503 | Abstract: | The increased incidence of obesity and related disorders in Western societies requires a thorough understanding of the adipogenic process. Data at the protein level of this process are scarce. Therefore we performed a proteome analysis of differentiating and starving 3T3L1 cells using two-dimensional gel electrophoresis combined with mass spectrometry. Effects of different starvation conditions were examined by subjecting 3T3-L1 adipocytes to caloric restriction, either in the absence or the presence of the lipolysis inducer tumor necrosis factor-alpha. Ninety-three differentially expressed proteins were found during differentiation and starvation of 3T3-L1 cells, 50 of which were identified. GenMAPP/ MAPPfinder software revealed a non-reciprocal regulation of the glycolytic pathway during 3T3-L1 differentiation followed by starvation. Furthermore, proteins involved in growth regulation, cytoskeletal rearrangements and protein modification, 16 of which have not been described before in 3T3-L1 cells, were identified. In conclusion, our data provide valuable information for further understanding of the adipogenic process. | Notes: | Maastricht Univ, NUTRIM, Maastricht Proteom Ctr, Dept Human Biol, NL-6200 MD Maastricht, Netherlands. Limburgs Univ Ctr, Biomed Res Inst, B-3590 Diepenbeek, Belgium. Transnatl Univ Limburg, Sch Life Sci, B-3590 Diepenbeek, Belgium. Tech Univ Eindhoven, BiGCaT Bioinformat, NL-6200 MD Maastricht, Netherlands. Maastricht Univ, NL-6200 MD Maastricht, Netherlands.Renes, J, Maastricht Univ, NUTRIM, Maastricht Proteom Ctr, Dept Human Biol, POB 616, NL-6200 MD Maastricht, Netherlands.j.renes@hb.unimaas.nl | Keywords: | 3T3-L1; proteomics; differentiation; caloric restriction; TNF-alpha | Document URI: | http://hdl.handle.net/1942/2123 | ISSN: | 1420-682X | e-ISSN: | 1420-9071 | DOI: | 10.1007/s00018-004-4498-9 | ISI #: | 000227163800009 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2006 |
Appears in Collections: | Research publications |
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