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DC Field | Value | Language |
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dc.contributor.author | KEUNEN, Els | - |
dc.contributor.author | Florez-Sarasa, I. | - |
dc.contributor.author | Obata, T. | - |
dc.contributor.author | JOZEFCZAK, Marijke | - |
dc.contributor.author | REMANS, Tony | - |
dc.contributor.author | VANGRONSVELD, Jaco | - |
dc.contributor.author | Fernie A.R. | - |
dc.contributor.author | CUYPERS, Ann | - |
dc.date.accessioned | 2016-05-23T13:50:35Z | - |
dc.date.available | 2016-05-23T13:50:35Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 124, p. 64-78 | - |
dc.identifier.issn | 0098-8472 | - |
dc.identifier.uri | http://hdl.handle.net/1942/21245 | - |
dc.description.abstract | The mitochondrial alternative oxidase (AOX) is suggested to modulate metabolic homeostasis and is activated at transcript and protein level in cadmium (Cd)-exposed Arabidopsis thaliana plants. In this study, the importance of AOX in the metabolic response to Cd stress was investigated. Primary metabolites were determined in roots and leaves of wild-type plants and aox1a knockout mutants exposed to sublethal Cd concentrations for 24 and 72 h. Additional parameters (gene expression, enzyme capacity) were included to support the results at the metabolic level. Whereas sugar and organic acid levels initially dropped in roots, the opposite was observed in leaves of both genotypes exposed to Cd. Regarding genotype-related differences in the leaves, our results strengthen the previously proposed link between AOX1a and ethylene. Furthermore, respiratory flux dependent on amino acid degradation could be related to AOX1a function in leaves of Cd-exposed plants. In roots, a relation between AOX1a and antioxidative defence – in particular glutathione (GSH) – is demonstrated during Cd exposure. In conclusion, A. thaliana plants show a tissue-specific response to sublethal Cd exposure, with a differential role for AOX1a in roots and leaves. | - |
dc.description.sponsorship | This work was supported by the Research Foundation Flanders (FWO) by a grant for Els Keunen and project [G0D3414]. Additional funding came from Hasselt University through the Methusalem project [08M03VGRJ]. Igor Florez-Sarasa was supported by a postdoc Fellowship of the Alexander von Humboldt Foundation. We thank Carine Put and Ann Wijgaerts for their skilful technical assistance. Seeds of aox1a knockout plants were kindly provided by Dr Chihiro Watanabe (Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Japan). Seeds of cad2-1 mutants were kindly donated by Dr Christopher Cobbett (Melbourne University, Australia). Seeds of rax1-1 mutants were kindly donated by Prof. Dr Phil Mullineaux (University of Essex, England). | - |
dc.language.iso | en | - |
dc.rights | © 2015 Elsevier B.V. All rights reserved. | - |
dc.subject.other | Alternative oxidase (AOX); arabidopsis thaliana; cadmium; metabolic profiling; oxidative challenge | - |
dc.title | Metabolic responses of Arabidopsis thaliana roots and leaves to sublethal cadmium exposure are differentially influenced by ALTERNATIVE OXIDASE1a | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 78 | - |
dc.identifier.spage | 64 | - |
dc.identifier.volume | 124 | - |
local.bibliographicCitation.jcat | A1 | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1016/j.envexpbot.2015.11.015 | - |
dc.identifier.isi | 000370907300005 | - |
item.accessRights | Restricted Access | - |
item.validation | ecoom 2017 | - |
item.fulltext | With Fulltext | - |
item.fullcitation | KEUNEN, Els; Florez-Sarasa, I.; Obata, T.; JOZEFCZAK, Marijke; REMANS, Tony; VANGRONSVELD, Jaco; Fernie A.R. & CUYPERS, Ann (2016) Metabolic responses of Arabidopsis thaliana roots and leaves to sublethal cadmium exposure are differentially influenced by ALTERNATIVE OXIDASE1a. In: ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 124, p. 64-78. | - |
item.contributor | KEUNEN, Els | - |
item.contributor | Florez-Sarasa, I. | - |
item.contributor | Obata, T. | - |
item.contributor | JOZEFCZAK, Marijke | - |
item.contributor | REMANS, Tony | - |
item.contributor | VANGRONSVELD, Jaco | - |
item.contributor | Fernie A.R. | - |
item.contributor | CUYPERS, Ann | - |
crisitem.journal.issn | 0098-8472 | - |
crisitem.journal.eissn | 1873-7307 | - |
Appears in Collections: | Research publications |
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1-s2.0-S0098847215300435-main.pdf Restricted Access | Published version | 2.94 MB | Adobe PDF | View/Open Request a copy |
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