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http://hdl.handle.net/1942/21359
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DC Field | Value | Language |
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dc.contributor.author | BECKERS, Bram | - |
dc.contributor.author | OP DE BEECK, Michiel | - |
dc.contributor.author | WEYENS, Nele | - |
dc.contributor.author | Van Acker, Rebecca | - |
dc.contributor.author | Van Montagu, Marc | - |
dc.contributor.author | Boerjan, Wout | - |
dc.contributor.author | VANGRONSVELD, Jaco | - |
dc.date.accessioned | 2016-06-01T08:08:48Z | - |
dc.date.available | 2016-06-01T08:08:48Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Proceedings of the National Academy of Sciences of the United States of America, 113 (8), p. 2312-2317 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/1942/21359 | - |
dc.description.abstract | Cinnamoyl-CoA reductase (CCR), an enzyme central to the lignin biosynthetic pathway, represents a promising biotechnological target to reduce lignin levels and to improve the commercial viability of lignocellulosic biomass. However, silencing of the CCR gene results in considerable flux changes of the general and monolignol-specific lignin pathways, ultimately leading to the accumulation of various extractable phenolic compounds in the xylem. Here, we evaluated host genotype-dependent effects of field-grown, CCR-down-regulated poplar trees (Populus tremula × Populus alba) on the bacterial rhizosphere microbiome and the endosphere microbiome, namely the microbiota present in roots, stems, and leaves. Plant-associated bacteria were isolated from all plant compartments by selective isolation and enrichment techniques with specific phenolic carbon sources (such as ferulic acid) that are up-regulated in CCR-deficient poplar trees. The bacterial microbiomes present in the endosphere were highly responsive to the CCR-deficient poplar genotype with remarkably different metabolic capacities and associated community structures compared with the WT trees. In contrast, the rhizosphere microbiome of CCRdeficient and WT poplar trees featured highly overlapping bacterial community structures and metabolic capacities. We demonstrate the host genotype modulation of the plant microbiome by minute genetic variations in the plant genome. Hence, these interactions need to be taken into consideration to understand the full consequences of plant metabolic pathway engineering and its relation with the environment and the intended genetic improvement. | - |
dc.description.sponsorship | This work was supported by Research Foundation-Flanders Project G032912N, Ghent University Multidisciplinary Research Partnership "Biotechnology for Sustainable Economy" Project 01MRB510W, and Hasselt University Bijzonder Onderzoeksfonds Methusalem Project 08M03VGRJ. M.O.D.B. and N.W. were a research fellow and postdoctoral fellow of the Research Foundation-Flanders. | - |
dc.language.iso | en | - |
dc.subject.other | host genotype modulation; CCR gene silencing; plant-associated bacteria | - |
dc.title | Lignin engineering in field-grown poplar trees affects the endosphere bacterial microbiome. | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 2317 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | 2312 | - |
dc.identifier.volume | 113 | - |
local.bibliographicCitation.jcat | A1 | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1073/pnas.1523264113 | - |
dc.identifier.isi | 000370620300088 | - |
item.fullcitation | BECKERS, Bram; OP DE BEECK, Michiel; WEYENS, Nele; Van Acker, Rebecca; Van Montagu, Marc; Boerjan, Wout & VANGRONSVELD, Jaco (2016) Lignin engineering in field-grown poplar trees affects the endosphere bacterial microbiome.. In: Proceedings of the National Academy of Sciences of the United States of America, 113 (8), p. 2312-2317. | - |
item.contributor | BECKERS, Bram | - |
item.contributor | OP DE BEECK, Michiel | - |
item.contributor | WEYENS, Nele | - |
item.contributor | Van Acker, Rebecca | - |
item.contributor | Van Montagu, Marc | - |
item.contributor | Boerjan, Wout | - |
item.contributor | VANGRONSVELD, Jaco | - |
item.validation | ecoom 2017 | - |
item.accessRights | Open Access | - |
item.fulltext | With Fulltext | - |
crisitem.journal.issn | 0027-8424 | - |
crisitem.journal.eissn | 1091-6490 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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PNAS-2016-Beckers-2312-7.pdf | Published version | 1.59 MB | Adobe PDF | View/Open |
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