Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2136
Title: A three-dimensional cryo-electron microscopy structure of the bacteriophage phi KZ head
Authors: Fokine, A
Kostyuchenko, VA
Efimov, AV
Kurochkina, LP
Sykilinda, NN
ROBBEN, Johan 
Volckaert, G
Hoenger, A
Chipman, PR
Battisti, A.J.
Rossmann, MG
Mesyanzhinov, Vadim V.
Issue Date: 2005
Publisher: ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
Source: JOURNAL OF MOLECULAR BIOLOGY, 352(1). p. 117-124
Abstract: The three-dimensional structure of the Pseudomonas aeruginosa bacteriophage Phi KZ head has been determined by cryo-electron microscopy and image reconstruction to 18 (A) over circle resolution. The head has icosahedral symmetry measuring 1455 (a) over circle in diameter along 5-fold axes and a unique portal vertex to which is attached an approximately 1800 (A) over circle -long contractile tail. The 65 kDa major capsid protein, gp120, is organized into a surface lattice of hexamers, with T=27 triangulation. The shape and size of the hexamers is similar to the hexameric building blocks of the bacteriophages T4, Phi 29, P22, and HK97. Pentameric vertices of the capsid are occupied by complexes composed of several special vertex proteins. The double-stranded genomic DNA is packaged into a highly condensed series of layers, separated by 24 (A) over circle, that follow the contour of the inner wall of the capsid. (c) 2005 Elsevier Ltd. All rights reserved.
Notes: Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. Shemyakin Ovchinnikov Inst Bioorgan Chem, Lab Mol Bioengn, Moscow 117997, Russia. Univ Hasselt, Biomed Res Inst, B-3590 Diepenbeek, Belgium. Katholieke Univ Leuven, Lab Gene Technol, B-3001 Louvain, Belgium. European Mol Biol Lab, Struct Programme, D-69117 Heidelberg, Germany.Rossmann, MG, Purdue Univ, Dept Biol Sci, 915 W State St, W Lafayette, IN 47907 USA.mr@purdue.edu
Keywords: bacteriophage phi KZ; capsid structure; cryo-electron microscopy; Pseudomonas aeruginosa; three-dimensional image reconstruction
Document URI: http://hdl.handle.net/1942/2136
ISSN: 0022-2836
e-ISSN: 1089-8638
DOI: 10.1016/j.jmb.2005.07.018
ISI #: 000231835000010
Category: A1
Type: Journal Contribution
Validations: ecoom 2006
Appears in Collections:Research publications

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