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http://hdl.handle.net/1942/21509
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DC Field | Value | Language |
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dc.contributor.author | Maes, Evelyne | - |
dc.contributor.author | Tirez, Kristof | - |
dc.contributor.author | Baggerman, Geert | - |
dc.contributor.author | VALKENBORG, Dirk | - |
dc.contributor.author | Schoofs, Liliane | - |
dc.contributor.author | Ruiz Encinar, Jorge | - |
dc.contributor.author | Mertens, Inge | - |
dc.date.accessioned | 2016-06-10T13:28:26Z | - |
dc.date.available | 2016-06-10T13:28:26Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | MASS SPECTROMETRY REVIEWS, 35 (3), p. 350-360 | - |
dc.identifier.issn | 0277-7037 | - |
dc.identifier.uri | http://hdl.handle.net/1942/21509 | - |
dc.description.abstract | Reversible phosphorylation is one of the most important post-translational modifications in mammalian cells. Because this molecular switch is an important mechanism that diversifies and regulates proteins in cellular processes, knowledge about the extent and quantity of phosphorylation is very important to understand the complex cellular interplay. Although phosphoproteomics strategies are applied worldwide, they mainly include only molecular mass spectrometry (like MALDI or ESI)-based experiments. Although identification and relative quantification of phosphopeptides is straightforward with these techniques, absolute quantification is more complex and usually requires for specific isotopically phosphopeptide standards. However, the use of elemental mass spectrometry, and in particular inductively coupled plasma mass spectrometry (ICP-MS), in phosphoproteomics-based experiments, allow one to absolutely quantify phosphopeptides. Here, these phosphoproteomic applications with ICP-MS as elemental detector are reviewed. Pioneering work and recent developments in the field are both described. Additionally, the advantage of the parallel use of molecular and elemental mass spectrometry is stressed. | - |
dc.description.sponsorship | The authors acknowledge the Flemish Institute for Technological Research (VITO). Evelyne Maes is funded by a Stichting Emmanuel van der Schueren research grant of the Vlaamse Liga tegen Kanker (VLK). | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.rights | © 2014 by Wiley Periodicals, Inc. | - |
dc.subject.other | phosphoproteomics; phosphorylation; ICP-MS; 31P; absolute quantification | - |
dc.subject.other | phosphoproteomics; phosphorylation; ICP-MS; P-31; absolute quantification | - |
dc.title | The use of elemental mass spectrometry in phosphoproteomic applications | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 360 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 350 | - |
dc.identifier.volume | 35 | - |
local.format.pages | 11 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Maes, Evelyne; Tirez, Kristof; Baggerman, Geert; Valkenborg, Dirk; Mertens, Inge] Flemish Inst Technol Res VITO, Boeretang 200, B-2400 Mol, Belgium. [Maes, Evelyne; Schoofs, Liliane] Katholieke Univ Leuven, Res Grp Funct Genom & Prote, Leuven, Belgium. [Baggerman, Geert; Valkenborg, Dirk; Mertens, Inge] Univ Antwerp, CFP CeProMa, B-2020 Antwerp, Belgium. [Valkenborg, Dirk] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Diepenbeek, Belgium. [Ruiz Encinar, Jorge] Univ Oviedo, Dept Phys & Analyt Chem, Oviedo, Spain. | - |
local.publisher.place | HOBOKEN | - |
local.type.refereed | Refereed | - |
local.type.specified | Review | - |
dc.identifier.doi | 10.1002/mas.21440 | - |
dc.identifier.isi | 000373733800002 | - |
item.fullcitation | Maes, Evelyne; Tirez, Kristof; Baggerman, Geert; VALKENBORG, Dirk; Schoofs, Liliane; Ruiz Encinar, Jorge & Mertens, Inge (2016) The use of elemental mass spectrometry in phosphoproteomic applications. In: MASS SPECTROMETRY REVIEWS, 35 (3), p. 350-360. | - |
item.fulltext | With Fulltext | - |
item.accessRights | Restricted Access | - |
item.contributor | Maes, Evelyne | - |
item.contributor | Tirez, Kristof | - |
item.contributor | Baggerman, Geert | - |
item.contributor | VALKENBORG, Dirk | - |
item.contributor | Schoofs, Liliane | - |
item.contributor | Ruiz Encinar, Jorge | - |
item.contributor | Mertens, Inge | - |
item.validation | ecoom 2017 | - |
crisitem.journal.issn | 0277-7037 | - |
crisitem.journal.eissn | 1098-2787 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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maes 1.pdf Restricted Access | Published version | 420.71 kB | Adobe PDF | View/Open Request a copy |
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