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Title: | Successful Treatment of Human Visceral Leishmaniasis Restores Antigen-Specific IFN-gamma, but not IL-10 Production | Authors: | Adem, Emebet Tajebe, Fitsumbirhan Getahun, Mulusew Kiflie, Amare Diro, Ermias Hailu, Asrat SHKEDY, Ziv Mengesha, Bewketu Mulaw, Tadele Atnafu, Saba Deressa, Tekalign Mathewos, Biniam Abate, Ebba Modolell, Manuel Munder, Markus Mueller, Ingrid Takele, Yegnasew Kropf, Pascale |
Issue Date: | 2016 | Publisher: | PUBLIC LIBRARY SCIENCE | Source: | PLoS neglected tropical diseases, 10 (3) | Abstract: | One of the key immunological characteristics of active visceral leishmaniasis (VL) is a profound immunosuppression and impaired production of Interferon-gamma (IFN-gamma). However, recent studies from Bihar in India showed using a whole blood assay, that whole blood cells have maintained the capacity to produce IFN-gamma. Here we tested the hypothesis that a population of low-density granulocytes (LDG) might contribute to T cell responses hyporesponsiveness via the release of arginase. Our results show that this population is affected by the anticoagulant used to collect blood: the frequency of LDGs is significantly lower when the blood is collected with heparin as compared to EDTA; however, the anticoagulant does not impact on the levels of arginase released. Next, we assessed the capacity of whole blood cells from patients with active VL to produce IFN-gamma and IL-10 in response to antigen-specific and polyclonal activation. Our results show that whole blood cells produce low or levels below detection limit of IFN-gamma and IL-10, however, after successful treatment of VL patients, these cells gradually regain their capacity to produce IFN-gamma, but not IL-10, in response to activation. These results suggest that in contrast to VL patients from Bihar, India, whole blood cells from VL patients from Gondar, Ethiopia, have lost their ability to produce IFN-gamma during active VL and that active disease is not associated with sustained levels of IL-10 production following stimulation. | Notes: | [Adem, Emebet; Tajebe, Fitsumbirhan; Getahun, Mulusew; Kiflie, Amare; Deressa, Tekalign; Mathewos, Biniam; Abate, Ebba] Univ Gondar, Dept Immunol, Gondar, Ethiopia. [Diro, Ermias] Univ Gondar, Dept Internal Med, Gondar, Ethiopia. [Hailu, Asrat] Univ Addis Ababa, Dept Microbiol Immunol & Parasitol, Addis Ababa, Ethiopia. [Shkedy, Ziv] Univ Hasselt, Dept Math & Stat, Diepenbeek, Belgium. [Mengesha, Bewketu; Mulaw, Tadele; Atnafu, Saba; Takele, Yegnasew] Gondar Univ, Leishmaniasis Res & Treatment Ctr, Gondar, Ethiopia. [Modolell, Manuel] Max Planck Inst Immunobiol & Epigenet, Dept Cellular Immunol, Freiburg, Germany. [Munder, Markus] Univ Med Ctr Mainz, Dept Med Hematol Oncol & Pneumol 3, Mainz, Germany. [Mueller, Ingrid; Takele, Yegnasew; Kropf, Pascale] Univ London Imperial Coll Sci Technol & Med, Dept Med, London, England. | Document URI: | http://hdl.handle.net/1942/21541 | ISSN: | 1935-2735 | e-ISSN: | 1935-2735 | DOI: | 10.1371/journal.pntd.0004468 | ISI #: | 000373272500016 | Rights: | Copyright: © 2016 Adem et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | Research publications |
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