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http://hdl.handle.net/1942/21559
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DC Field | Value | Language |
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dc.contributor.author | LOUWIES, Tijs | - |
dc.contributor.author | Vuegen, Caroline | - |
dc.contributor.author | INT PANIS, Luc | - |
dc.contributor.author | COX, Bianca | - |
dc.contributor.author | VRIJENS, Karen | - |
dc.contributor.author | NAWROT, Tim | - |
dc.contributor.author | DE BOEVER, Patrick | - |
dc.date.accessioned | 2016-06-27T10:12:56Z | - |
dc.date.available | 2016-06-27T10:12:56Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | ENVIRONMENTAL RESEARCH, 147, p. 24-31 | - |
dc.identifier.issn | 0013-9351 | - |
dc.identifier.uri | http://hdl.handle.net/1942/21559 | - |
dc.description.abstract | Introduction: Air pollution, a risk factor for cardiovascular diseases, can exert its effects through the microcirculation. Retinal blood vessel width is considered a marker for microvascular health and is associated with short-term PM10 exposure. microRNAs are key regulators of complex biological processes in cardiovascular health and disease and miRNA expression can be affected by air pollution exposure. Studies investigating the effect of ambient air pollution exposure on miRNA expression in combination with an assessment of the microvasculature do not exist. Methods: 50 healthy adults (50% women, 23-58 years old) were examined once a month from December 2014 until April 2015 in Flanders (Belgium). Fundus photos and venous blood samples were collected during the study visits. PM10, data were obtained from a nearby monitoring station. Image analysis was used to calculate the width of retinal blood vessels, represented as the Central Retinal Arteriolar/Venular Equivalent (CRAE/CRVE). Total miRNA was isolated from blood and the expression of miR-21, -146a and, -222 were measured using quantitative real-time PCR. Mixed models were used for statistical analysis. Results: Each short-term increase of 10 mu g/m(3) PM10 during the 24 h preceding the study visit was associated with a 0.58 mu m decrease (95% CI: -1.16, -0.0005; p=0.056) in CRAE, a 0.99 mu m increase (95% CI: 0.18, 1.80; p=0.021) in CRVE, a 6.6% decrease (95% CI: -11.07, -2.17; p=0.0038) in miR-21 expression and a 6.7% decrease (95% CI: -10.70, -2.75; p=0.0012) in miR-222 expression. Each 10% increase in miR-21 was associated with a 0.14 mu m increase (95% CI: 0.0060, 0.24; p=0.046) in CRAE whereas a similar increase in miR-222 expression was associated with a 0.28 mu m decrease (95% CI: -0.50, -0.062; p=0.016) in CRVE. These associations were also found in exposure windows ranging from 2 h to 1 week. Finally, we observed that the association between PM10, exposure and CRAE was mediated by miRNA-21 expression. Conclusion: PM10 exposure was associated with retinal arteriolar narrowing and venular widening. M PM10 exposure affected miRNAs that are involved in inflammatory and oxidative stress pathways. We suggest that miRNA changes may be relevant to explain the association between PM10 and retinal vessel calibers. (C) 2016 Elsevier Inc. All rights reserved. | - |
dc.description.sponsorship | Tijs Louwies is supported with a VITO PhD fellowship. Karen Vrijens is a postdoctoral researcher of the Research Foundation Flanders (FWO). Tim S. Nawrot is holder of an European Research Council starting grant (ERC-2012-StG 310898). | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.rights | © 2016 Elsevier Inc. All rights reserved. | - |
dc.subject.other | air pollution; retina; microvascular changes; miRNA expression | - |
dc.subject.other | Air pollution; Retina; Microvascular changes; miRNA expression | - |
dc.title | miRNA expression profiles and retinal blood vessel calibers are associated with short-term particulate matter air pollution exposure | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 31 | - |
dc.identifier.spage | 24 | - |
dc.identifier.volume | 147 | - |
local.format.pages | 8 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Louwies, Tijs; Panis, Luc Int; De Boever, Patrick] Flemish Inst Technol Res VITO, Environm Risk & Hlth, Mol, Belgium. [Louwies, Tijs; Vuegen, Caroline; Cox, Bianca; Vrijens, Karen; Nawrot, Tim S.; De Boever, Patrick] Hasselt Univ, Ctr Environm Sci, Hasselt, Belgium. [Panis, Luc Int] Hasselt Univ, Transportat Res Inst, Hasselt, Belgium. [Nawrot, Tim S.] Leuven Univ, Dept Publ Hlth & Primary Care, Leuven, Belgium. | - |
local.publisher.place | SAN DIEGO | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1016/j.envres.2016.01.027 | - |
dc.identifier.isi | 000374275700004 | - |
item.validation | ecoom 2017 | - |
item.contributor | LOUWIES, Tijs | - |
item.contributor | Vuegen, Caroline | - |
item.contributor | INT PANIS, Luc | - |
item.contributor | COX, Bianca | - |
item.contributor | VRIJENS, Karen | - |
item.contributor | NAWROT, Tim | - |
item.contributor | DE BOEVER, Patrick | - |
item.accessRights | Restricted Access | - |
item.fullcitation | LOUWIES, Tijs; Vuegen, Caroline; INT PANIS, Luc; COX, Bianca; VRIJENS, Karen; NAWROT, Tim & DE BOEVER, Patrick (2016) miRNA expression profiles and retinal blood vessel calibers are associated with short-term particulate matter air pollution exposure. In: ENVIRONMENTAL RESEARCH, 147, p. 24-31. | - |
item.fulltext | With Fulltext | - |
crisitem.journal.issn | 0013-9351 | - |
crisitem.journal.eissn | 1096-0953 | - |
Appears in Collections: | Research publications |
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