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Title: Regenerative endodontic procedure of an infected immature permanent human tooth: an immunohistological study
Authors: Meschi, Nastaran
Van den Eynde, Kathleen
Mavridou, Athina
Strijbos, Olaf
De Ketelaere, Marieke
Van Gorp, Gertrude
Lambrechts, Paul
Issue Date: 2016
Source: CLINICAL ORAL INVESTIGATIONS, 20 (4), p. 807-814
Abstract: Objectives An immunohistological study of an infected immature permanent human tooth after a regenerative endodontic procedure (REP) was conducted in order to determine the histologic outcome of this procedure. Besides observed signs of angiogenesis and neurogenesis, repair and/or regeneration of the pulp-dentin complex was also investigated. Materials and methods A REP was performed on tooth 45 of a 10-year-old girl. Eleven months post-treatment, the tooth had to be removed for orthodontic reasons. The following investigations were performed: immunohistology and radiographic quantification of root development. After hematoxylin-eosin (HE) staining, the following immunomarkers were selected: neurofilament (NF), pan cytokeratin (PK), osteocalcin (OC), and CD34. Results The REP resulted in clinical and radiographic healing of the periradicular lesion and quantifiable root development. The HE staining matches with the medical imaging post-REP: underneath the mineral trioxide aggregate a calcified bridge with cell inclusions, connective pulp-like tissue (PLT) with blood vessels, osteodentin against the root canal walls, on the root surface cementum (Ce), and periodontal ligament (PDL). The PDL was PK+ . The blood vessels in the PLT and PDL were CD34+ . The Ce, osteodentin, and stromal cells in the PLT were OC+ . The neurovascular bundles in the PLT were NF+ . Conclusions Immunohistologically, REP of this infected immature permanent tooth resulted in an intracanalar connective tissue with a regulated physiology, but not pulp tissue. Clinical relevance REP of an immature permanent infected tooth may heal the periapical infection and may result in a combination of regeneration and repair of the pulp-dentin complex.
Notes: [Meschi, Nastaran; Mavridou, Athina; Strijbos, Olaf; Van Gorp, Gertrude; Lambrechts, Paul] KU Leuven & Dent, Univ Hosp Leuven, Dept Oral Hlth Sci, Leuven, Belgium. [Hilkens, Petra; Lambrichts, Ivo] Hasselt Univ, Biomed Res Inst BIOMED, Lab Morphol, B-3590 Diepenbeek, Belgium. [Van den Eynde, Kathleen] Katholieke Univ Leuven, Dept Imaging & Pathol, Lab Translat Cell & Tissue Res, Leuven, Belgium. [De Ketelaere, Marieke] H Horriestr 40 Bus 13, B-8800 Roeselare, Belgium.
Keywords: Angiogenesis; Pulp biology; Stem cell(s); Tooth development;angiogenesis; pulp biology; stem cell(s); tooth development
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ISSN: 1432-6981
e-ISSN: 1436-3771
DOI: 10.1007/s00784-015-1555-8
ISI #: 000374562300020
Rights: © Springer-Verlag Berlin Heidelberg 2015
Category: A1
Type: Journal Contribution
Validations: ecoom 2017
Appears in Collections:Research publications

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