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http://hdl.handle.net/1942/22075
Title: | Imaging of transfection and intracellular release of intact, non-labeled DNA using fluorescent nanodiamonds | Authors: | Petrakova, V. Benson, V. Buncek, M. Fiserova, A. Ledvina, M. Stursa, J. Cigler, P. NESLADEK, Milos |
Issue Date: | 2016 | Publisher: | ROYAL SOC CHEMISTRY | Source: | NANOSCALE, 8(23), p. 12002-12012 | Abstract: | Efficient delivery of stabilized nucleic acids (NAs) into cells and release of the NA payload are crucial points in the transfection process. Here we report on the fabrication of a nanoscopic cellular delivery carrier that is additionally combined with a label-free intracellular sensor device, based on biocompatible fluorescent nanodiamond particles. The sensing function is engineered into nanodiamonds by using nitrogen-vacancy color centers, providing stable non-blinking luminescence. The device is used for monitoring NA transfection and the payload release in cells. The unpacking of NAs from a poly(ethyleneimine)-terminated nanodiamond surface is monitored using the color shift of nitrogen-vacancy centers in the diamond, which serve as a nanoscopic electric charge sensor. The proposed device innovates the strategies for NA imaging and delivery, by providing detection of the intracellular release of non-labeled NAs without affecting cellular processing of the NAs. Our system highlights the potential of nanodiamonds to act not merely as labels but also as non-toxic and non-photobleachable fluorescent bio-sensors reporting complex molecular events. | Notes: | [Petrakova, V.; Benson, V.; Fiserova, A.; Ledvina, M.; Nesladek, M.] Czech Tech Univ, Fac Biomed Engn, Sitna Sq 3105, Kladno 27201, Czech Republic. [Petrakova, V.] Inst Phys AS CR, Vvi, Slovance 1999-2, Prague 18221 8, Czech Republic. [Benson, V.; Fiserova, A.] Inst Microbiol AS CR, Vvi, Videnska 1083, Prague 14220 4, Czech Republic. [Buncek, M.] Generi Biotech Ltd, Machkova 587, Hradec Kralove 50011, Czech Republic. [Ledvina, M.; Cigler, P.] Inst Organ Chem & Biochem AS CR, Vvi, Flemingovo Nam 2, Prague 16610 6, Czech Republic. [Stursa, J.] Nucl Phys Inst AS CR, Vvi, Rez 25068, Czech Republic. [Nesladek, M.] Hasselt Univ, IMEC Div IMOMEC, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. [Nesladek, M.] Hasselt Univ, Inst Mat Res, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. | Document URI: | http://hdl.handle.net/1942/22075 | ISSN: | 2040-3364 | e-ISSN: | 2040-3372 | DOI: | 10.1039/c6nr00610h | ISI #: | 000378510700029 | Rights: | This journal is © The Royal Society of Chemistry 2016 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2017 |
Appears in Collections: | Research publications |
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c6nr00610h.pdf | Published version | 2.44 MB | Adobe PDF | View/Open |
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